Reproductive BioMedicine Online
Volume 19, Issue 6 , Pages 770-777 , December 2009

Phosphatidylserine translocation in human spermatozoa from impaired spermatogenesis

  • C Almeida

      Affiliations

    • Department of Genetics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
    • Corresponding Author InformationCorrespondence:
    • ,
  • M Sousa

      Affiliations

    • Department of Genetics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
    • Centre for Reproductive Genetics Alberto Barros, 4100-009 Porto, Portugal
    • Laboratory of Cell Biology, ICBAS-Institute of Biomedical Sciences Abel Salazar, University of Porto, 1099-003 Porto, Portugal
    • ,
  • A Barros

      Affiliations

    • Department of Genetics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
    • Centre for Reproductive Genetics Alberto Barros, 4100-009 Porto, Portugal

Received 27 February 2009 ,Revised 30 March 2009 ,Accepted 8 September 2009.

References 

  1. Almeida C, Cardoso MF, Sousa M, et al. Quantitative study of caspase-3 activity in semen and after swim-up preparation in relation to sperm quality. Human Reproduction. 2005;20:1307–1313
  2. Alvarez JG. Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular sperm. Human Reproduction. 2005;20:2031–2032author reply 2032–2033
  3. Alvarez JG. Nurture vs Nature: how can we optimize sperm quality?. Journal of Andrology. 2003;24:640–648
  4. Barroso G, Taylor S, Morshedi M, et al. Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: a comparison of two different sperm populations. Fertility and Sterility. 2006;85:149–154
  5. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Human Reproduction. 2000;15:1338–1344
  6. Eguchi J, Koji T, Nomata K, et al. Fas–Fas ligand system as a possible mediator of spermatogenic cell apoptosis in human maturation-arrested testes. Human Cell. 2002;15:61–68
  7. Ferrás C, Fernandes S, Marques CJ, et al. AZF and DAZ gene copy-specific deletion analysis in maturation arrest and Sertoli cell-only syndrome. Molecular Human Reproduction. 2004;10:755–761
  8. Francavilla S, D’Abrizio P, Cordeschi G, et al. Fas expression correlates with human germ cell degeneration in meiotic and post-meiotic arrest of spermatogenesis. Molecular Human Reproduction. 2002;8:213–220
  9. Francavilla S, D’Abrizio P, Rucci N, et al. Fas and Fas ligand expression in fetal and adult human testis with normal or deranged spermatogenesis. Journal of Clinical Endocrinology and Metabolism. 2000;85:2692–2700
  10. Greco E, Scarselli F, Iacobelli M, et al. Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Human Reproduction. 2005;20:226–230
  11. Kierszenbaum AL. Apoptosis during spermatogenesis: the thrill of being alive. Molecular Reproduction and Development. 2001;58:1–3
  12. Kim SK, Yoon YD, Park YS, et al. Involvement of the Fas–Fas ligand system and active caspase-3 in abnormal apoptosis in human testes with maturation arrest and Sertoli cell-only syndrome. Fertility and Sterility. 2007;87:547–553
  13. Lachaud C, Tesarik J, Cañadas ML, Mendoza C. Apoptosis and necrosis in human ejaculated spermatozoa. Human Reproduction. 2004;19:607–610
  14. Lin WW, Lamb DJ, Wheeler TM, et al. In situ end-labelling of human testicular tissue demonstrates increased apoptosis in conditions of abnormal spermatogenesis. Fertility and Sterility. 1997;68:1065–1069
  15. Marchetti C, Gallego MA, Defossez A, et al. Staining of human sperm with fluorochrome-labelled inhibitor of caspases to detect activated caspases: correlation with apoptosis and sperm parameters. Human Reproduction. 2004;19:1127–1134
  16. Martin G, Sabido O, Durand P, Levy R, et al. Phosphatidylserine externalization in human sperm induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction. Human Reproduction. 2005;20:3459–3468
  17. Martin SJ, Reutelingsperger CP, McGahon AJ, et al. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. Journal of Experimental Medicine. 1995;182:1545–1556
  18. Naito M, Nagashima K, Mashima T, Tsuruo T, et al. Phosphatidylserine externalization is a downstream event of Interleukin-1β-Converting enzyme family protease activation during apoptosis. Blood. 1997;89:2060–2066
  19. Nakanishi Y, Shiratsuchi A. Phagocytic removal of apoptotic spermatogenic cells by Sertoli cells: mechanisms and consequences. Biological and Pharmaceutical Bulletin. 2004;27:13–16
  20. Oosterhuis GJ, Mulder AB, Kalsbeek-Batenburg E, et al. Measuring apoptosis in human spermatozoa: a biological assay for semen quality. Fertility and Sterility. 2000;74:245–250
  21. Pentikainen V, Erkkila K, Dunkel L. Fas regulates germ cell apoptosis in the human testis in vitro. American Journal of Physiology. 1999;276:E310–E316
  22. Pinho MJ, Neves R, Costa P, et al. Unique t(Y;1)(q12;q12) reciprocal translocation with loss of the heterochromatic region of chromosome 1 in a male with azoospermia due to meiotic arrest: a case report. Human Reproduction. 2005;20:689–696
  23. Print CG, Loveland KL. Germ cell suicide: new insights into apoptosis during spermatogenesis. Bioessays. 2000;22:423–430
  24. Ramos L, Kleingeld P, Meuleman E, et al. Assessment of DNA fragmentation of spermatozoa that were surgically retrieved from men with obstructive azoospermia. Fertility and Sterility. 2002;77:233–237
  25. Ricci G, Perticarari S, Fragonas E, et al. Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes. Human Reproduction. 2002;17:2665–2672
  26. Rodriguez I, Ody C, Araki K, Garcia I, Vassalli P, et al. An early and massive wave of germinal cell apoptosis is required for the development of functional spermatogenesis. EMBO Journal. 1997;16:2262–2270
  27. Shen HM, Dai J, Chia SE, Lim A, Ong CN, et al. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Human Reproduction. 2002;17:1266–1273
  28. Shiratsuchi A, Umeda M, Ohba Y, Nakanishi Y, et al. Recognition of phosphatidylserine on the surface of apoptotic spermatogenic cells and subsequent phagocytosis by Sertoli cells of the rat. Journal of Biological Chemistry. 1997;272:2354–2358
  29. Sousa M, Cremades N, Silva J, et al. Predictive value of testicular histology in secretory azoospermic subgroups and clinical outcome after microinjection of fresh and frozen-thawed sperm and spermatids. Human Reproduction. 2002;17:1800–1810
  30. Takagi S, Itoh N, Kimura M, Sasao T, Tsukamoto T, et al. Spermatogonial proliferation and apoptosis in hypospermatogenesis associated with nonobstructive azoospermia. Fertility and Sterility. 2001;76:901–907
  31. Taylor SL, Weng SL, Fox P, et al. Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality. Molecular Human Reproduction. 2004;10:825–834
  32. Tesarik J, Ubaldi F, Rienzi L, et al. Caspase-dependent and -independent DNA fragmentation in Sertoli and germ cells from men with primary testicular failure: relationship with histological diagnosis. Human Reproduction. 2004;2:254–261
  33. Tesarik J, Greco E, Mendoza C. Assisted reproduction with in-vitro-cultured testicular spermatozoa in cases of severe germ cell apoptosis: a pilot study. Human Reproduction. 2001;16:2640–2645
  34. Tesarik J, Greco E, Cohen-Bacrie P, Mendoza C, et al. Germ cell apoptosis in men with complete and incomplete spermiogenesis failure. Molecular Human Reproduction. 1998;4:757–762
  35. Van den Eijnde SM, Boshart L, Reutelingsperger CP, et al. Phosphatidylserine plasma membrane asymmetry in vivo: a pancellular phenomenon which alters during apoptosis. Cell Death and Differentiation. 1997;4:311–316
  36. Van Engeland M, Nieland LJ, Ramaekers FC, et al. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry. 1998;31:1–9
  37. Varum S, Bento C, Sousa AP, et al. Characterization of human sperm populations using conventional parameters, surface ubiquitination, and apoptotic markers. Fertility and Sterility. 2007;87:572–583
  38. Wang X, Sharma RK, Sikka SC, et al. Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. Fertility and Sterility. 2003;80:531–535
  39. Weng SL, Taylor SL, Morshedi M, et al. Caspase activity and apoptotic markers in ejaculated human sperm. Molecular Human Reproduction. 2002;8:984–991
  40. World Health Organization 1999 Laboratory Manual for the Examination of Human Semen and Sperm Cervical Mucus Interaction. Cambridge University Press, UK.

 Carolina Almeida obtained her degree in biology in 1997 from the University of Porto, Portugal. She has been working in the area of human genetics, including the study of male and female infertility and some genetic diseases, since 2002. At present, and simultaneously with a laboratory position, she is finishing her PhD at the Department of Genetics of the Faculty of Medicine, University of Porto.Carolina Almeida

PII: S1472-6483(09)00144-8

doi: 10.1016/j.rbmo.2009.10.002

Reproductive BioMedicine Online
Volume 19, Issue 6 , Pages 770-777 , December 2009

Article Tools

* Image Usage & Resolution