Reproductive BioMedicine Online
Volume 20, Issue 2 , Pages 201-208 , February 2010

Ultra-rapid vitrification of mouse oocytes in low cryoprotectant concentrations

  • Ho-Joon Lee

      Affiliations

    • Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    • Ho-Joon Lee and Heidi Elmoazzen contributed equally to this work
    • Corresponding Author InformationCorresponding authors.
    • ,
  • Heidi Elmoazzen

      Affiliations

    • Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA 02114, USA
    • Ho-Joon Lee and Heidi Elmoazzen contributed equally to this work
    • ,
  • Diane Wright

      Affiliations

    • Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    • ,
  • John Biggers

      Affiliations

    • Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    • ,
  • Bo R Rueda

      Affiliations

    • Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    • ,
  • Yun Seok Heo

      Affiliations

    • Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA 02114, USA
    • ,
  • Mehmet Toner

      Affiliations

    • Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, Boston, MA 02114, USA
    • ,
  • Thomas L Toth

      Affiliations

    • Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    • Corresponding Author InformationCorresponding authors.

Received 26 May 2009 ,Revised 4 June 2009 ,Accepted 2 November 2009.

References 

  1. American Society for Reproductive Medicine, Practice Committee of Society for Assisted Reproductive Technology 2008 Ovarian tissue and oocyte cryopreservation. Fertil. Steril. 90 (Suppl. 5), S241–S246.
  2. Berejnov V, Husseini NS, Alsaied OA, Thorne RE. Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions. J. Appl. Crystallogr. 2006;39:244–251
  3. Bernard A, Fuller BJ. Cryopreservation of human oocytes: a review of current problems and perspectives. Hum. Reprod. Update. 1996;2:193–207
  4. Biggers JD, McGinnis LK, Raffin M. Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biol. Reprod. 2000;63:281–293
  5. Chen SU, Lien YR, Chao KH, et al. Effects of cryopreservation on meiotic spindles of oocytes and its dynamics after thawing: clinical implications in oocyte freezing – a review article. Mol. Cell. Endocrinol. 2003;202:101–107
  6. Elmoazzen HY, Poovadan A, Law GK, et al. Dimethyl sulfoxide toxicity kinetics in intact porcine articular cartilage. Cell Tissue Banking. 2007;6:125–133
  7. Eroglu A, Toth TL, Toner M. Alterations of the cytoskeleton and polyploidy induced by cryopreservation of metaphase II mouse oocytes. Fertil. Steril. 1998;69:944–957
  8. Fahy GM, Macfarlane DR, Angell CA, Meryman HT. Vitrification as an approach to cryopreservation. Cryobiology. 1984;21:407–426
  9. Fosas N, Marina F, Torres PJ, et al. The births of five Spanish babies from cryopreserved donated oocytes. Hum. Reprod. 2003;18:1417–1421
  10. Gook DA, Edgar DH. Human oocyte cryopreservation. Hum. Reprod. Update. 2007;13:591–605
  11. Handyside AH, Hunter S. A rapid procedure for visualizing the inner cell mass and trophectoderm nuclei of mouse blastocysts in situ using polynucleotide-specific fluorochromes. J. Exp. Zool. 1984;231:429–434
  12. He XM, Park EY, Fowler A, et al. Vitrification by ultra-fast cooling at a low concentration of cryoprotectants in a quartz micro-capillary: a study using murine embryonic stem cells. Cryobiology. 2008;56:223–232
  13. Isachenko V, Alabart JL, Nawroth F, et al. The open pulled straw vitrification of ovine GV-oocytes: positive effect of rapid cooling or rapid thawing or both?. Cryo-Letters. 2001;22:157–162
  14. Johari GP, Hallbrucker A, Mayer E. The glass liquid transition of hyperquenched water. Nature. 1987;330:552–553
  15. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 2007;67:73–80
  16. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod. Biomed. Online. 2005;11:300–308
  17. Lane M, Bavister BD, Lyons EA, Forest KT. Containerless vitrification of mammalian oocytes and embryos – adapting a proven method for flash-cooling protein crystals to the cryopreservation of live cells. Nat. Biotechnol. 1999;17:1234–1236
  18. Lawitts JA, Biggers JD. Culture of preimplantation embryos. Methods Enzymol. 1993;225:153–164
  19. Ludwig M, Al-Hasani S, Felberbaum R, Diedrich K. New aspects of cryopreservation of oocytes and embryos in assisted reproduction and future perspectives. Hum. Reprod. 1999;14:162–185
  20. Luyet B. On Various Phase transitions occurring in aqueous solutions at low temperatures. Ann. N. Y. Acad. Sci. 1960;85:549–569
  21. Martino A, Songsasen N, Leibo SP. Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol. Reprod. 1996;54:1059–1069
  22. Mazur P. Freezing of living cells – mechanisms and implications. Am. J. Physiol. 1984;247:C125–C142
  23. Mazur P, Rall WF, Leibo SP. Kinetics of water-loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature-dependence of water permeability. Cell Biophys. 1984;6:197–213
  24. Mazur P, Cole KW, Schreuders PD, Mahowald AP. Contributions of cooling and warming rate and developmental stage to the survival of Drosophila embryos cooled to −205°C. Cryobiology. 1993;30:45–73
  25. Meirow D. Reproduction post-chemotherapy in young cancer patients. Mol. Cell. Endocrinol. 2000;169:123–131
  26. Nagy A, Rossant J, Nagy R, et al. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl Acad. Sci. USA. 1993;90:8424–8428
  27. Noyes N, Porcu E, Borini A. Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomalies. Reprod. Biomed. Online. 2009;18:769–776
  28. Porcu E, Fabbri R, Seracchioli R, et al. Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil. Steril. 1997;68:724–726
  29. Quinn P, Moinipanah R, Steinberg JM, Weathersbee PS. Successful human in-vitro fertilization using a modified human tubal fluid medium lacking glucose and phosphate ions. Fertil. Steril. 1995;63:922–924
  30. Ragni G, Allegra A, Anserini P, et al. The 2004 Italian legislation regulating assisted reproduction technology: a multicentre survey on the results of IVF cycles. Hum. Reprod. 2005;20:2224–2228
  31. Risco R, Elmoazzen H, Doughty M, et al. Thermal performance of quartz capillaries for vitrification. Cryobiology. 2007;55:222–229
  32. Sjostrand FS, Elfvin LG. Granular structure of mitochondrial membranes and of cytomembranes as demonstrated in frozen-dried tissue. J. Ultrastruct. Res. 1964;10:263
  33. Steponkus PL, Myers SP, Lynch DV, et al. Cryopreservation of Drosophila melanogaster embryos. Nature. 1990;345:170–172
  34. Tucker MJ, Wright G, Morton PC, Massey JB. Birth after cryopreservation of immature oocytes with subsequent in-vitro maturation. Fertil. Steril. 1998;70:578–579
  35. Vajta G, Holm P, Kuwayama M, et al. Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev. 1998;51:53–58
  36. Whittingham DG, Leibo SP, Mazur P. Survival of mouse embryos frozen to −196 and −269°C. Science. 1972;178:411
  37. Yavin S, Aroyo A, Roth Z, Arav A. Embryo cryopreservation in the presence of low concentration of vitrification solution with sealed pulled straws in liquid nitrogen slush. Hum. Reprod. 2009;24:797–804
  38. Yoon TK, Lee DR, Cha SK, et al. Survival rate of human oocytes and pregnancy outcome after vitrification using slush nitrogen in assisted reproductive technologies. Fertil. Steril. 2007;88:952–956

 Ho-Joon Lee has been working in the area of reproductive biology and medicine since 1985, beginning with a human IVF program and as a director of the IVF lab in Cheil General Hospital, Seoul, Korea. He was awarded a PhD in Hanyang University, Seoul, Korea, 1993. He developed co-culture and embryo cryopreservation techniques to improve pregnancy rates. He joined the Vincent Center for Reproductive Biology in Massachusetts General Hospital in 2003, where he is currently working as an investigator. His current interests are oocyte and embryo preservation and he focuses on ovarian stem cells in mammal as well as fertility preservation.

PII: S1472-6483(09)00225-9

doi: 10.1016/j.rbmo.2009.11.012

Reproductive BioMedicine Online
Volume 20, Issue 2 , Pages 201-208 , February 2010

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