Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI
Received 1 June 2009; received in revised form 20 July 2009; accepted 19 November 2009. published online 22 January 2010.
Abstract
Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful use of this novel technique applied to a highly apoptotic semen sample before performing intracytoplasmic sperm injection (ICSI) is reported here. The use of annexin V microbeads for selecting non-apoptotic spermatozoa seems to reduce the percentage of altered cells, improving the chance of pregnancy after ICSI.
Declaration: The authors report no financial or commercial conflicts of interest.
aCentro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina
bLaboratory of Testicular Physiology and Pathology, Center for Research in Endocrinology, Buenos Aires Children’s Hospital, Argentina
Corresponding author.
Vanesa Rawe is the director of the Laboratory of Research and Special Studies (LABINEE) at Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina. She graduated from the University of Buenos Aires and received a PhD in cellular biology of reproduction. She obtained an advanced fellowship in reproductive sciences for postdoctoral training at the University of Pittsburgh Medical School directed by Dr Schatten. Her research has examined aspects of the biology of fertilization especially in humans. Her current interests focus on the effects of sperm and oocyte pathology on the fertilization processes and advancing technologies surrounding gamete interactions.
ABSTRACT
Vanesa Rawe is the director of the Laboratory of Research and Special Studies (LABINEE) at Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina. She graduated from the University of Buenos Aires and received a PhD in cellular biology of reproduction. She obtained an advanced fellowship in reproductive sciences for postdoctoral training at the University of Pittsburgh Medical School directed by Dr Schatten. Her research has examined aspects of the biology of fertilization especially in humans. Her current interests focus on the effects of sperm and oocyte pathology on the fertilization processes and advancing technologies surrounding gamete interactions.