Reproductive BioMedicine Online
Volume 20, Issue 3 , Pages 320-323, March 2010

Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI

  • Vanesa Y. Rawe

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina
    • Corresponding Author InformationCorresponding author.
    • ,
  • Heydy Uriondo Boudri

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina
    • ,
  • Cristian Alvarez Sedó

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina
    • Laboratory of Testicular Physiology and Pathology, Center for Research in Endocrinology, Buenos Aires Children’s Hospital, Argentina
    • ,
  • Martina Carro

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina
    • ,
  • Sergio Papier

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina
    • ,
  • Florencia Nodar

      Affiliations

    • Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina

Received 1 June 2009; received in revised form 20 July 2009; accepted 19 November 2009. published online 22 January 2010.

Declaration: The authors report no financial or commercial conflicts of interest.

Abstract 

Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful use of this novel technique applied to a highly apoptotic semen sample before performing intracytoplasmic sperm injection (ICSI) is reported here. The use of annexin V microbeads for selecting non-apoptotic spermatozoa seems to reduce the percentage of altered cells, improving the chance of pregnancy after ICSI.

Keywords: annexin V microbeads, apoptosis, DNA fragmentation, ICSI, MACS, spermatozoa

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 Vanesa Rawe is the director of the Laboratory of Research and Special Studies (LABINEE) at Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina. She graduated from the University of Buenos Aires and received a PhD in cellular biology of reproduction. She obtained an advanced fellowship in reproductive sciences for postdoctoral training at the University of Pittsburgh Medical School directed by Dr Schatten. Her research has examined aspects of the biology of fertilization especially in humans. Her current interests focus on the effects of sperm and oocyte pathology on the fertilization processes and advancing technologies surrounding gamete interactions.

PII: S1472-6483(09)00276-4

doi:10.1016/j.rbmo.2009.12.004

Reproductive BioMedicine Online
Volume 20, Issue 3 , Pages 320-323, March 2010

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