Reproductive BioMedicine Online
Volume 20, Issue 3 , Pages 371-379 , March 2010

Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment

  • Csaba Pribenszky

      Affiliations

    • Department of Animal Breeding and Genetics, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary
    • Corresponding Author InformationCorresponding author.
  • ,
  • Eszter Losonczi

      Affiliations

    • ARTechnic Co. Research and Development, Csapó u. 13, Debrecen, Hungary
  • ,
  • Miklós Molnár

      Affiliations

    • ARTechnic Co. Research and Development, Csapó u. 13, Debrecen, Hungary
  • ,
  • Zsolt Lang

      Affiliations

    • Department of Biomathematics and Informatics, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest 1078, Hungary
  • ,
  • Szabolcs Mátyás

      Affiliations

    • Department of Embryology, KAÁLI Institute, Istenhegyi út 54/A, Budapest 1125, Hungary
  • ,
  • Klára Rajczy

      Affiliations

    • Department of Embryology, KAÁLI Institute, Istenhegyi út 54/A, Budapest 1125, Hungary
  • ,
  • Katalin Molnár

      Affiliations

    • Department of Embryology, KAÁLI Institute, Istenhegyi út 54/A, Budapest 1125, Hungary
  • ,
  • Péter Kovács

      Affiliations

    • Department of Embryology, KAÁLI Institute, Istenhegyi út 54/A, Budapest 1125, Hungary
  • ,
  • Péter Nagy

      Affiliations

    • Department of Electronics Technology, Faculty of Electrical Engineering and Informatics, Budapest University of Technology and Economics, Budapest, Hungary
  • ,
  • Jason Conceicao

      Affiliations

    • PIVET Medical Centre, 166–168 Cambridge St., Perth, WA, Australia
  • ,
  • Gábor Vajta

      Affiliations

    • Cairns Fertility Centre, 58–60 McLeod St., Cairns, Qld 4870, Australia

Received 6 July 2009 ,Revised 3 August 2009 ,Accepted 26 November 2009.

References 

  1. Agresti A. Categorical Data Analysis. second ed.. Wiley; 2002;pp. 491–537
  2. Alikani M. The origins and consequences of fragmentation in mammalian eggs and embryos. In:  Elder K,  Cohen J editor. Human Preimplantation Embryo Selection. London: Informa Healthcare; 2007;p. 51–78
  3. Alikani M, Calderon G, Tomkin G, et al. Cleavage anomalies in early human embryos and survival after prolonged culture in vitro. Hum. Reprod. 2000;15:2634–2643
  4. Arav A, Aroyo A, Yavin S, et al. Prediction of embryonic developmental competence by time-lapse observation and ‘shortest half’ analysis. Reprod. Biomed. Online. 2008;17:669–675
  5. Bavister BD. A mini-chamber device for maintaining a constant carbon dioxidein air atmosphere during prolonged culture of cells on the stage of an inverted microscope. In Vitro Cell Dev. Biol. 1988;24:759–763
  6. Bhattacharya S, Templeton A. What is the most relevant standard of success in assisted reproduction? Redefining success in the context of elective single embryo transfer: evidence, intuition and financial reality. Hum. Reprod. 2004;19:1939–1942
  7. Booth PJ, Watson TJ, Leese HJ. Prediction of porcine blastocyst formation using morphological, kinetic, and amino acid depletion and appearance criteria determined during the early cleavage of in vitro-produced embryos. Biol. Reprod. 2007;77:765–779
  8. Bos-Mikich A, Mattos ALG, Ferrari AN. Early cleavage of human embryos: an effective method for predicting successful IVF/ICSI outcome. Hum. Reprod. 2001;16:2658–2661
  9. Brison DR, Houghton FD, Falconer D, Roberts SA, Hawkhead J, Humpherson PG, et al. Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover. Hum. Reprod. 2004;19:2319–2324
  10. Cole RJ. Cinemicrographic observations on the trophoblast and zona pellucida of the mouse blastocyst. J. Embryol. Exp. Morphol. 1967;17:481–490
  11. Cummins JM, Breen TM, Harrison KL, et al. A formula for scoring human embryo growth rates in in-vitro fertilization: its value in predicting pregnancy and in comparison with visual estimates of embryo quality. J. In Vitro Fertilization Embryo Transfer. 1986;3:284–295
  12. Ebner T, Yaman C, Moser M, et al. Embryo fragmentation in vitro and its impact on treatment and pregnancy outcome. Fertil. Steril. 2001;76:281–285
  13. Edwards R, Fishel S, Cohen J. Factors influencing the success of in vitro fertilization for alleviating human infertility. J. In Vitro Fertilization Embryo Transfer. 1984;1:3–23
  14. Emiliani S, Fasano G, Vandamme B, et al. Impact of the assessment of early cleavage in a single embryo transfer policy. Reprod. Biomed. Online. 2006;13:255–260
  15. Fenwick J, Platteau P, Mucdoch AP, et al. Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro. Hum. Reprod. 2002;17:407–412
  16. Gardner DK, Lane M. Towards a single embryo transfer. Reprod. Biomed. Online. 2003;6:470–481
  17. Giorgettic C, Hans E, Terriou P, et al. Early cleavage: an additional predictor of high implantation rate following elective single embryo transfer. Reprod. Biomed. Online. 2007;14:85–91
  18. Gopichandran N, Leese HJ. The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos. Reproduction. 2006;131:269–277
  19. Hardarson T, Löfman C, Coull G, et al. Internalization of cellular fragments in a human embryo: time-lapse recordings. Reprod. Biomed. Online. 2002;5:36–38
  20. Harper J, Robinson F, Duffy S. Detection of fertilization in embryos with accelerated cleavage by fluorescence in situ hybridization. Hum. Reprod. 1994;9:1733–1737
  21. Hesters L, Prisant N, Fanchin R, et al. Impact of early cleaved zygote morphology on embryo development and in vitro fertilization–embryo transfer outcome: a prospective study. Fertil. Steril. 2008;89:1677–1684
  22. Holm P, Booth PJ, Callesen H. Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media. Reproduction. 2002;123:553–565
  23. Katz-Jaffe MG, McReynolds S, Gardner DK, et al. The role of proteomics in defining the human embryonic secretome. Mol. Hum. Reprod. 2009;(Epub ahead of print)
  24. Lemmen JG, Agerholm I, Ziebe S. Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI-fertilized oocytes. Reprod. Biomed. Online. 2008;17:385–391
  25. Loutradi KE, Kolibianakis EM, Venetis CA, et al. Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil. Steril. 2008;90:186–193
  26. Luna M, Copperman AB, Duke M, et al. Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (>or =10 cells), bringing into question current embryological dogma. Fertil. Steril. 2008;89:358–363
  27. Lundin, K., Bergh, C., Hardarson, T., 2001. Early embryo cleavage is a strong indicator of embryo quality. In: Third Biennial Alpha Conference, vol. 3 (Suppl. 1), New York City, New York, USA, p. 52 (abstract).
  28. Magli M, Gianaroli L, Munne S, et al. Incidence of chromosomal abnormalities from a morphological normal cohort of embryos in poor prognosis patients. J. Assist. Reprod. Genet. 1998;15:297–301
  29. Massip A, Mulnard J. Time-lapse cinematographic analysis of hatching of normal and frozen–thawed cow blastocysts. J. Reprod. Fertil. 1980;58:475–478
  30. Montag M, van der Ven H. Symposium: innovative techniques in human embryo viability assessment. Oocyte assessment and embryo viability prediction: birefringence imaging. Reprod. Biomed. Online. 2008;17:454–460
  31. Montfoort A, Dumoulin J, et al. Early cleavage is a valuable addition to existing embryo selection parameters: a study using single embryo transfers. Hum. Reprod. 2004;19:2103–2108
  32. Nagy ZP, Jones-Colon S, Roos P, et al. Metabolomic assessment of oocyte viability. Reprod. Biomed. Online. 2009;18:219–225
  33. Pandian Z, Templeton A, Serour G, et al. Number of embryos for transfer after IVF and ICSI: a Cochrane review. Hum. Reprod. 2005;20:2681–2687
  34. Petersen CG, Mauri AL, Ferreira R, et al. Embryo selection by the first cleavage parameter between 25 and 27h after ICSl. J. Assist. Reprod. Genet. 2001;18:209–212
  35. Quea G, Romero K, Garcia-Velasco JA. Extended embryo culture to increase implantation rate. Reprod. Biomed. Online. 2007;14:375–383
  36. Ragione T, Verheyen G, Papanikolaou EG, et al. Developmental stage on day-5 and fragmentation rate on day-3 can influence the implantation potential of top-quality blastocysts in IVF cycles with single embryo transfer. Reprod. Biol. Endocrinol. 2007;5:2
  37. Rienzi L, Ubaldi F, Iacobelli M, et al. Significance of morphological attributes of the early embryo. Reprod. Biomed. Online. 2005;10:669–681
  38. Scott L, Berntsen J, Davies D, et al. Symposium: innovative techniques in human embryo viability assessment. Human oocyte respiration-rate measurement – potential to improve oocyte and embryo selection?. Reprod. Biomed. Online. 2008;17:461–469
  39. Singh R, Sinclair KD. Metabolomics: approaches to assessing oocyte and embryo quality. Theriogenology. 2007;68S:S56–S62
  40. Stokes PJ, Abeydeera LR, Leese HJ. Development of porcine embryos in vivo and in vitro; evidence for embryo ‘cross talk’ in vitro. Dev. Biol. 2005;284:62–71
  41. Sturmey RG, Brison DR, Leese HJ. Symposium: innovative techniques in human embryo viability assessment. Assessing embryo viability by measurement of amino acid turnover. Reprod. Biomed. Online. 2008;17:486–496
  42. Vajta G, Peura TT, Holm P, et al. New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system. Mol. Reprod. Dev. 2000;55:256–264
  43. Vajta G, Kőrösi T, Du Y, et al. The Well-of-the-Well system: an efficient approach to improve embryo development. Reprod. Biomed. Online. 2008;17:73–81
  44. Van Blerkom J, Davis P, Alexander S. A microscopic and biochemical study of fragmentation phenotypes in stage-appropriate human embryos. Hum. Reprod. 2001;16:719–729
  45. Windt M-L, Krueger TF, Coetzee K, et al. Comparative analysis of pregnancy rates after the transfer of early dividing embryos versus slower dividing embryos. Hum. Reprod. 2004;19:1155–1162
  46. Wright RW, Anderson GB, Cupps PT, Drost M. Blastocyst expansion and hatching of bovine ova cultured in vitro. J. Anim. Sci. 1976;43:170–174
  47. Xie Y, Wang F, Zhong W, et al. Shear stress induces preimplantation embryo death that is delayed by the zona pellucida and associated with stress-activated protein kinase-mediated apoptosis. Biol. Reprod. 2006;75:45–55
  48. Youssry M, Ozmen B, Zohni K, et al. Current aspects of blastocyst cryopreservation. Reprod. Biomed. Online. 2008;16:311–320
  49. Ziebe S, Peterson K, Lindenberg S, et al. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in vitro fertilization. Hum. Reprod. 1997;12:1545–1549

 Csaba Pribenszky obtained a DVM degree (1998) and a PhD degree (2005) in the field of assisted reproductive technologies in Budapest, Hungary. Together with Mikos Molnar, he developed and patented the concept of ‘stress for stress tolerance’, utilizing sub-lethal hydrostatic pressure stress treatment to gametes, embryos, other cells and tissues in order to improve cell survival during subsequent procedures (e.g. cryopreservation, SCNT). His current focus is the application of the above method to human oocyte vitrification and the effect of different environmental factors on in-vitro embryo developmental dynamics and embryo fragmentation.

PII: S1472-6483(09)00284-3

doi: 10.1016/j.rbmo.2009.12.007

Reproductive BioMedicine Online
Volume 20, Issue 3 , Pages 371-379 , March 2010