Reproductive BioMedicine Online
Volume 20, Issue 4 , Pages 453-469 , April 2010

Embryo culture: can we perform better than nature?

  • Gábor Vajta

      Affiliations

    • Cairns Fertility Centre, Cairns, QLD 4870, Australia
    • Corresponding Author InformationCorresponding author.
    • Current address: James Cook University, Cairns, QLD 4870, Australia.
    • ,
  • Laura Rienzi

      Affiliations

    • G.EN.E.R.A centre for Reproductive Medicine, Clinica Valle Giulia, Rome, Italy
    • ,
  • Ana Cobo

      Affiliations

    • IVI Universidad de Valencia Plaza de la Policía local, 3 46015 Valencia, Spain
    • ,
  • John Yovich

      Affiliations

    • Cairns Fertility Centre, Cairns, QLD 4870, Australia
    • PIVET Medical Centre, 166-168 Cambridge Street, Perth, WA 6007, Australia

Received 19 May 2009 ,Revised 20 July 2009 ,Accepted 9 December 2009.

References 

  1. Abramczuk J, Solter D, Koprowski H. The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium. Develop. Biol. 1977;61:378–383
  2. Bavister BD. Co-culture for embryo development: is it really necessary?. Human Reprod. 1992;7:1339–1341
  3. Bavister BD. Culture of preimplantation embryos: facts and artifact. Human Reprod. Update. 1995;1:91–148
  4. Bavister BD. How animal embryo research led to the first documented human IVF. Reprod. BioMed. Online. 2002;4(Suppl. 1):24–29
  5. Beebe DJ, Wheeler M, Zeringue H, et al. Microfluidic technology for assisted reproduction. Theriogenology. 2002;57:125–135
  6. Betteridge KJ, Rieger D. Embryo transfer and related techniques in domestic animals, and their implications for human medicine. Human Reprod. 1993;8:147–167
  7. Biggers JD. Ethical issues and the commercialization of embryo culture media. Reprod. BioMed. Online. 2000;1:74–76
  8. Biggers JD. Thoughts on embryo culture condition. Reprod. BioMed. Online. 2001;4:30–38
  9. Biggers JD, Summers MC. Choosing a culture medium: making informed choices. Fertil. Steril. 2008;90:473–483
  10. Biggers JD, McGinnis LK, Lawitts JA. One-step versus two-step culture of mouse preimplantation embryos: is there a difference?. Human Reprod. 2005;20:3376–3384
  11. Biggers JD, McGinnis LK, Summers MC. Dicrepancies between the effects of glutamine in cultures of preimplantation mouse embryos. Reprod. BioMed. Online. 2004;9:70–73
  12. Biggers JD, McGinnis LK, Summers MC. Reply: one step versus two-step culture of mouse preimplantation embryos. Human Reprod. 2006;21:1936–1939
  13. Booth PJ, Humpherson PG, Watson TJ, et al. Amino acid depletion and appearance during porcine preimplantation embryo development in vitro. Reproduction. 2005;130:655–668
  14. Booth PJ, Tan SJ, Reipurth R, et al. Simplification of bovine somatic cell nuclear transfer by application of a zona-free manipulation technique. Cloning Stem Cells. 2001;3:139–150
  15. Carolan C, Lonergan P, Khatir H, et al. In vitro production of bovine embryos using individual oocytes. Mol. Reprod. Dev. 1996;45:145–150
  16. Chang AS, Moley KH, Wangler M, et al. Association between Beckwith–Wiedemann syndrome and assisted reproductive technology: a case series of 19 patients. Fertil. Steril. 2005;83:349–354
  17. Conaghan J, Handyside AH, Winston RM, Leese HJ. Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J. Reprod. Fertil. 1993;99:87–95
  18. Desai N, Abdelhafez F, Bedaiwy MA, et al. Live births in poor prognosis IVF patients using a novel non-contact human endometrial co-culture system. Reprod. Biomed. Online. 2008;16:869–874
  19. Deter RL. Quantitative morphological analysis of early mouse embryogenesis in vitro. J. Embryol. Exper. Morphol. 1977;40:91–100
  20. Dominguez F, Gadea B, Mercader A, et al. Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequential system. Fertil. Steril. 2008;34:45–56December 3 [Epub ahead of print]
  21. Donnay I, Van Langendonckt A, Auquier P, et al. Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology. 1997;47:1549–1561
  22. Du Y, Lin L, Schmidt M, Bøgh IB, et al. High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. Cloning Stem Cells. 2008;10:325–330
  23. Du Y, Pribenszky CS, Molnár M, et al. High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification. Reproduction. 2008;135:13–17
  24. Duranthon V, Watson AJ, Lonergan P. Preimplantation embryo programming: transcription, epigenetics, and culture environment. Reproduction. 2008;135:141–150
  25. Ecker DJ, Stein P, Xu Z, et al. Long-term effects of culture of preimplantation mouse embryos on behaviour. Proc. National Acad. Sci. USA. 2004;101:1595–1600
  26. Eckert J, Pugh PA, Thompson JG, et al. Exogenous protein affects developmental competence and metabolic activity of bovine preimplantation embryos in vitro. Reprod., Fertil. Develop. 1999;10:327–332
  27. Edwards RG. Test-tube babies. Nature. 1981;293:253–256
  28. Feltrin C, Forell F, dos Santos L, et al. In vitro bovine embryo development after nuclear transfer by handmade cloning using a modified WOW culture system. Reprod., Fertil. Develop. 2006;18:126
  29. Fernandez-Gonzalez R, Moreira P, Bilbao A, et al. Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behaviour. Proc. National Acad. Sci. USA. 2004;101:5880–5885
  30. Gandolfi F. Autocrine, paracrine and environmental factors influencing embryonic development from zygote to blastocyst. Theriogenology. 1994;41:95–100
  31. Gardner DK. Dissection of culture media for embryos: the most important and less important components and characteristics. Reprod., Fertil. Develop. 2008;20:9–18
  32. Gardner DK, Lane M. Amino acids and ammonium regulate mouse embryo development in culture. Biol. Reprod. 1993;48:377–385
  33. Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF?. Human Reprod. Update. 1997;3:367–382
  34. Gardner DK, Lane M. Blastocyst transfer. Clin. Obstet. Gynaecol. 2003;46:231–238
  35. Gardner DK, Lane M. Culture of the mammalian preimplantation embryo. In:  Gardner DK,  Lane M,  Watson AJ editor. A Laboratory Guide to the Mammalian Embryo. Oxford, New York: Oxford University Press; 2004;p. 41–61
  36. Gardner DK, Lane M. One-step versus two-step culture of mouse preimplantation embryos. Human Reproduction. 2006;21:1935–1936
  37. Gardner DK, Lane M. Embryo culture systems. In:  Gardner DK editors. In Vitro Fertilization: A Practical Approach. New York, USA: Informa Healthcare; 2007;p. 221–282
  38. Gardner DK, Lane M, Batt PA. Uptake and metabolism of pyruvate and glucose by individual sheep pre-attachment embryos developed in vivo. Mol. Reprod. Develop. 1993;36:313–339
  39. Gardner DK, Lane M, Calderon I, et al. Environment of the preimplantation human embryo in vivo: Metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil. Steril. 1996;65:349–353
  40. Ghanem N, Hoelker M, Phatsara C, et al. Effect of well in well culture of bovine embryos on gene expression profile. Reprod., Fertil. Develop. 2009;21:190–191
  41. Gopichandran N, Leese HJ. The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos. Reproduction. 2006;131:269–277
  42. Hagemann LJ, Weilert LL, Beaumont SE, et al. Development of bovine embryos in single in vitro production (sIVP) system. Mol. Reprod. Develop. 1998;51:123–147
  43. Holm P, Booth PJ, Schmidt MH, et al. High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myoinositol with or without serum. Theriogenology. 1999;52:683–700
  44. Houghton FD, Hawkhead JA, Humpherson PA, et al. Non-invasive amino acid turnover predicts human embryo developmental capacity. Human Reprod. 2002;17:999–1005
  45. Hunter RHF. The Fallopian tubes in domestic mammals: how vital is their physiological activity?. Reprod., Nutrition Develop. 2005;45:281–290
  46. Hunter RHF, Bogh IB, Einter-Jensen N, et al. Pre-ovulatory graafian follicles are cooler than neighbouring stroma in pig ovaries. Human Reprod. 2000;15:273–283
  47. Hunter RHF, Einer-Jensen N, Greve T. Presence and significance of temperature gradients among different ovarian tissues. Microsc. Res. Tech. 2006;69:501–507
  48. Hunter RHF, Nichol R. A preovulatory temperature gradient between the isthmus and ampulla of pig oviducts during the phase of sper storage. J. Reprod. Fertil. 1986;77:599–606
  49. Hunter RHF, Nichol R, Leese HJ. Fallopian tube microenvironments, gametes and early embryo development. Havemeyer Found. Monogr. Ser. 2003;10:3–4
  50. Isom SC, Lai L, Prather RS, et al. Heat shock of porcine zygotes immediately after oocyte activation increases viability. Mol. Reprod. Develop. 2009;76:548–554
  51. Kattal N, Cohen J, Barmat LI. Role of coculture in human in vitro fertilization: a meta-analysis. Fertil. Steril. 2008;90:1069–1076
  52. Khosla S, Dean W, Brown D, et al. Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes. Biol. Reprod. 2001;64:918–926
  53. Khosla S, Dean W, Reik W, et al. Culture of preimplantation embryos and its long-term effects on gene expression and phenotype. Human Reprod. Update. 2001;7:419–427
  54. Kuo, Y.H., Pribenszky, C., Huang, S.Y., 2007. Higher litter size is achieved by the insemination of high hydrostatic pressure-treated frozen-thawed boar semen. In: Proceedings of the 6th International Conference on Boar Semen Preservation, Alliston, Ontario, Canada, p. III–22.
  55. Kültz D. Evolution of the cellular stress proteome: from monophyletic origin to ubiquitous function. J. Exper. Biol. 2003;206(Pt 18):3119–3124
  56. Kültz D. Molecular and evolutionary basis of the cellular stress response. Annu. Rev. Physiol. 2005;67:225–257
  57. Kwong WY, Wild AE, Roberts P, et al. Maternal undernutrition during the preimplantation period of rat development causes blastocyst abnormalities and programming of postnatal hypertension. Development. 2000;127:4195–4202
  58. Lagutina I, Lazzari G, Duchi R, et al. Comparative aspects of somatic cell nuclear transfer with conventional and zona-free methods in cattle, horse, pig and sheep. Theriogenology. 2007;67:90–98
  59. Lane M, Gardner DK. Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Human Reprod. 1992;7:558–562
  60. Lane M, Gardner DK. Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions. J. Reprod. Fertil. 1994;102:305–312
  61. Lane M, Gardner DK. Amino acids increase mouse embryo viability. Theriogenology. 1994;41:233
  62. Lane M, Gardner DK. Inhibiting 3-phosphoglycerate kinase by EDTA stimulates the development of the cleavage stage mouse embryos. Mol. Reprod. Develop. 2001;60:233–240
  63. Lane M, Gardner DK. Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse. Biol. Reprod. 2003;69:1109–1117
  64. Lane M, Gardner DK. Embryo culture medium: which is the best?. Best Practice Res. Clin. Obst. Gynaecol. 2007;21:83–100
  65. Lawrence LT, Moley KH. Epigenetics and assisted reproductive technologies: human imprinting syndromes. Seminars Reprod. Med. 2008;26:143–152
  66. Leach RE, Stachecki JJ, Armant DR. Development of in vitro fertilized mouse embryos exposed to ethanol during the preimplantation period: accelerated embryogenesis at subtoxic levels. Teratology. 1993;47:57–64
  67. Lee ES, Pugh PA, Allen N. [5–3H]-glucose and [1–14C]-pyruvate utilization by fresh and frozen-thawed Day 7 IVP bovine blastocysts cultured in PVA-or BSA-supplemented medium. Theriogenology. 1998;49:233
  68. Leese HJ. Human embryo culture: back to nature. J. Assisted Reprod. Genetics. 1998;15:466–468
  69. Leese HJ. Quiet please, do not disturb: a hypothesis of embryo metabolism and viability. Bioessays. 2002;24:845–849
  70. Leese HJ. What does an embryo need?. Human Fertil. (Camb.). 2003;6:180–185
  71. Leese HJ, Barton AM. Pyruvate and glucose uptake by mouse ova and preimplantation embryos. J. Reprod. Fertil. 1984;72:9–13
  72. Leese HJ, Conaghan J, Martin KL, et al. Early human embryo metabolism. Bioessays. 1993;15:259–264
  73. Leese HJ, Baumann CG, Brison DR, et al. Metabolism of the viable mammalian embryo: quietness revisited. Mol. Human Reprod. 2008;14:667–672
  74. Leese HJ, Hugentobler SA, Gray S. Female reproductive tract fluids: composition, mechanism of formation and potential role in the developmental origins of health and disease. Reprod., Fertil. Develop. 2008;20:1–8
  75. Leese HJ, Tay JI, Reischl J, et al. Formation of fallopian tubal fluid: role of a neglected epithelium. Reproduction. 2001;121:339–346
  76. Lin L, Du Y, Liu Y, et al. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes. Reprod. BioMed. Online. 2009;18:360–366
  77. Lin L, Kragh PM, Purup S, et al. Osmotic stress induced by sodium chloride sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes. Reprod., Fertil. Develop. 2009;21:338–344
  78. Lonergan P, Fair T, Corcoran D, et al. Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos. Theriogenology. 2006;65:137–152
  79. McLaren A, Biggers JD. Successful development and birth of mice cultivated in vitro as early as early embryos. Nature. 1958;182:877–878
  80. Meintjes M, Chantilis SJ, Douglas JD, et al. A controlled randomized trial evaluating the effect of lowered incubator oxygen tension on live births in a predominantly blastocyst transfer program. Human Reprod. 2009;24:300–307
  81. Meintjes M, Chantilis SJ, Ward DC. A randomized controlled study of human serum albumin and serum substitute supplement as protein supplements for IVF culture and the effect on live birth rates. Human Reprod. 2009;24:782–799
  82. Mendes PM. Stimuli-responsive surfaces for bio-applications. Chem. Soc. Rev. 2008;37:2512–2529
  83. Ménézo YJ, Hérubel F. Mouse and bovine models for human IVF. Reprod. BioMed. Online. 2002;4:170–175
  84. Mercader A, Valbuena D, Simón C. Human embryo culture. Methods Enzymol. 2006;420:3–18
  85. Miller AO, Menozzi FD, Dubois D. Microbeads and anchorage-dependent eukaryotic cells: the beginning of a new era in biotechnology. Adv. Biochem. Eng./Biotechnol. 1989;39:73–95
  86. Mortimer D. Elaboration of a new culture medium for physiological studies on human sperm motility and capacitation. Human Reprod. 1986;1:247–250
  87. Mortimer D. Human blastocyst development media. Human Reprod. 2001;16:2725
  88. Nagao Y, Saeki K, Hoshi M, et al. Effects of oxygen concentration and oviductal epithelial tissue on the development of in vitro matured and fertilized bovine oocytes cultured in protein-free medium. Theriogenology. 1994;41:681–687
  89. Oh SJ, Gong SP, Lee ST, et al. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. Fertil. Steril. 2007;88(4 Suppl.):1150–1157
  90. Oliver MH, Jacquiery AL, Bloomfield FH. The effects of maternal nutrition around the time of conception on the health of the offspring. J. Reprod. Fertil. 2007;64(Suppl.):397–410
  91. O’Neill C. Evidence for the requirement of autocrine growth factors for development of mouse preimplantation embryos in vitro. Biol. Reprod. 1997;56:229–237
  92. O’Neill C. The potential roles for embryotrophic ligands in preimplantation embryo development. Human Reprod. Update. 2008;14:275–288
  93. Otsuki J, Nagai Y, Chiba K. Peroxidation of mineral oil used in droplet culture is detrimental to fertilization and embryo development. Fertil. Steril. 2007;88:741–743
  94. Otsuki J, Nagai Y, Chiba K. Damage of embryo development caused by peroxidized mineral oil and its association with albumin in culture. Fertil. Steril. 2009;91:1745–1749
  95. Paoloni-Giacobino A. Genetic and epigenetic risks of ART. Fertil. Steril. 2007;88:761–762
  96. Parikh FR, Nadkarni SG, Naik NJ, et al. Cumulus coculture and cumulus-aided embryo transfer increases pregnancy rates in patients undergoing in vitro fertilization. Fertil. Steril. 2006;86:839–847
  97. Peura TT, Vajta G. A comparison of established and new approaches in ovine and bovine nuclear transfer. Cloning Stem Cells. 2003;5:257–277
  98. Pool TB. An update on embryo culture for human assisted reproductive technology: media, performance, and safety seminars. Reprod. Med. 2002;23:309–318
  99. Pool TB. Recent advances in the production of viable human embryos in vitro. Reprod. BioMed. Online. 2005;4:294–302
  100. Pribenszky C, Molnár M, Cseh S, et al. Improving post-thaw survival of cryopreserved mouse blastocysts by hydrostatic pressure challenge. Animal Reprod. Sci. 2005;87:143–150
  101. Pribenszky C, Molnár M, Horváth A. Hydrostatic pressure induced increase in post-thaw motility of frozen boar spermatozoa. Reprod., Fertil. Develop. 2005;18:162–163
  102. Pribenszky C, Molnár M, Ulrich P, et al. Pressure assisted cryopreservation: a novel possibility for IVP bovine blastocyst cryopreservation. Reprod. Domestic Animals. 2005;40:338
  103. Pribenszky C, Molnár M, Horváth A, et al. Improved post-thaw motility, viability and fertility are achieved by hydrostatic pressure treated bull semen. Reprod., Fertil. Develop. 2007;19:181–182
  104. Pribenszky C, Siquiera E, Molnár M, et al. Improved post-warming developmental competence of open pulled straw-vitrified in vitro-produced bovine blastocysts by sublethal hydrostatic pressure pre-treatment. Reprod., Fertil. Develop. 2007;20:125
  105. Pribenszky C, Du Y, Molnár M, et al. Increased stress tolerance of pig oocytes after high hydrostatic pressure treatment. Animal Reprod. Sci. 2008;106:200–207
  106. Pribenszky C, Molnár M, Kútvölgyi G, et al. Sublethal stress treatment of fresh boar semen with high hydrostatic pressure, inserted into the routine insemination procedure may improve average litter size by 1 piglet. Reprod. Domestic Animals. 2008;43:108
  107. Quinn P. Enhanced results in mouse and human embryo culture using a modified human tubal fluid medium lacking glucose and phosphate. J. Assisted Reprod. Gen. 1995;12:97–105
  108. Quinn P. Review of media used in ART laboratories. J. Androl. 2000;21:610–615
  109. Quinn P. The development and impact of culture media for assisted reproductive technologies. Fertil. Steril. 2004;81:27–29
  110. Quinn P, Horstman FC. Is the mouse a good model for the human with respect to the development of the preimplantation embryo in vitro?. Human Reprod. 1998;13(Suppl. 4):173–183
  111. Quinn P, Kerin JF, Warnes GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil. Steril. 1985;44:493–498
  112. Rahman PK, Pasirayi G, Auger V, et al. Development of a simple and low cost microbioreactor for high-throughput bioprocessing. Biotechnol. Lett. 2009;31:209–214
  113. Richter KS. The importance of growth factors for preimplantation embryo development and in-vitro culture. Curr. Opin. Obst. Gynecol. 2008;20:292–304
  114. Rieger, D., Schimmel, T., Cohen, J., et al., 2007. Comparison of GPS and standard dishes for embryo culture: set-up and observation times, and embryo development. In: Proceedings of the 14th World Congress on IVF and 3rd World Congress on IVM, Montreal, p. 1202.
  115. Roh S, Choi Y-J, Min B-M. A novel microtube culture system that enhances the in vitro development of parthenogenetic murine embryos. Theriogenology. 2008;69:262–267
  116. Rooke JA, McEvoy TG, Ashworth CJ. Ovine fetal development is more sensitive to perturbation by the presence of serum in embryo culture before rather than after compaction. Theriogenology. 2007;67:639–647
  117. Schini SA, Bavister BD. Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol. Reprod. 1988;39:1183–1192
  118. Selye H. A syndrome produced by diverse nocuous agents. 1936. J. Neuropsych. Clin. Neurosci. 1998;10:230–231
  119. Sepúlveda S, Garcia J, Arriaga E, et al. In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system. Fertil. Steril. 2008;91:1765–1770
  120. Shettles LB. Observations on human follicular and tubal ova. Am. J. Obst. Gynecol. 1953;66:235–247
  121. Silver L: Human-animal Chimeras: From Mythology To Biotechnology. In Scientific Blogging, Science 2.0, 2007. <http://www.scientificblogging.com/lee_silver/human_animal_chimeras_from_mythology_to_biotechnology>.
  122. Sinclair KD. Assisted reproductive technologies and pregnancy outcomes: mechanistic insights from animal studies. Seminars Reprod. Med. 2008;26:153–161
  123. Smith, A.U., 1951. Fertilisation in vitro of the mammalian egg. In: Biochemical Society Symposia (Cambridge), vol. 7 (cit. Bavister, 2002), pp. 3–10.
  124. Smith GD, Takayama S. Gamete and embryo isolation and culture with microfluidics. Theriogenology. 2007;68S:S190–S195
  125. Steeves TE, Gardner DK. Temporal and differential effects of amino acids on bovine embryo development in culture. Biol. Reprod. 1999;61:731–740
  126. Summers MC, Biggers JD. Chemically defined media and culture of mammalian preimplantation embryos: historical perspective and current issues. Human Reprod. Update. 2003;9:557–582
  127. Tay JL, Rutheford AJ, Killick SR, et al. Human tubal fluid: production, nutrient composition and response to adrenergic agents. Human Reprod. 1997;12:2451–2456
  128. Thompson JG. Defining the requirements for bovine embryo culture. Theriogenology. 1996;45:27–40
  129. Thompson JG. In vitro culture and embryo metabolism of cattle and sheep embryos – a decade of achievement. Animal Reprod. Sci. 2000;60–61:263–275
  130. Thompson JG. Culture without the Petri dish. Theriogenology. 2007;67:16–20
  131. Thompson JG, Peterson AJ. Bovine embryo culture in vitro: new developments and post-transfer consequences. Human Reprod. 2000;15(Suppl. 5):59–67
  132. Thompson JG, Gardner DK, Pugh PA, et al. Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos. Biol. Reprod. 1995;53:1385–1391
  133. Thompson JG, Mitchell M, Kind KL. Embryo culture and long-term consequences. Reprod. Fertil. Develop. 2007;19:43–52
  134. Thouas GA, Jones GM, Trounson AO. The ‘GO’ system–a novel method of microculture for in vitro development of mouse zygotes to the blastocyst stage. Reproduction. 2003;126:161–169
  135. Trounson A, Conti A. Research in human in-vitro fertilisation and embryo transfer. Brit. Med. J. (Clin. Res. Ed.). 1982;285:244–248
  136. Trounson A, Pushett D, Maclellan LJ, et al. Current status of IVM/IVF and embryo culture in humans and farm animals. Theriogenology. 1994;41:57–66
  137. Vajta G, Alexopoulos NI, Callesen H. Rapid growth and elongation of bovine blastocysts in vitro in a three-dimensional gel system. Theriogenology. 2004;62:1253–1263
  138. Vajta G, Holm P, Greve T, et al. The submarine incubation system, a new tool for in vitro embryo culture. a technique report. Theriogenology. 1997;48:1379–1385
  139. Vajta G, Holm P, Kuwayama M, et al. Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Develop. 1998;51:53–58
  140. Vajta G, Kőrösi T, Du Y, et al. The Well-of-the-Well system: an efficient approach to improve embryo development. Reprod. BioMed. Online. 2008;17:73–81
  141. Vajta G, Kragh PM, Mtango NR, et al. Handmade cloning approach: potentials and limitations. Reprod., Fertil. Develop. 2005;17:97–112
  142. Vajta G, Lewis IM, Hyttel P, et al. Somatic cell cloning without micromanipulators. Cloning. 2001;3:89–95
  143. Vajta G, Peura TT, Holm P, et al. New method for culture of zona-included and zone-free embryos: the Well of the Well (WOW) system. Mol. Reprod. Develop. 2000;55:256–264
  144. Vajta G, Zhang Y, Macháty Z. Somatic cell nuclear transfer in pig: recent achievements and future possibilities. Reprod., Fertil. Develop. 2007;19:403–423
  145. Van Wagtendonk-de Leeuw AM, Aerts Bj, den Daas JH. Abnormal offspring following in vitro production of bovine preimplantation embryos: a field study. Theriogenology. 1998;49:883–894
  146. Virant-Klun I, Tomazevic T, Vrtacnik-Bogal E, et al. Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage. Fertil. Steril. 2006;85:526–528
  147. Vodicka P, Smetana K, Dvoránková B, et al. The miniature pig as an animal model in biomedical research. Ann. New York Acad. Sci. 2005;1049:161–171
  148. Voelkel SA, Hu YX. Effect of gas atmosphere on the development of one-cell bovine embryos in two culture systems. Theriogenology. 1992;37:1117–1131
  149. Wemekamp-Kamphuis HH, Karatzas AK, et al. Enhanced levels of cold shock proteins in Listeria Monocitygenes LO28 upon exposure to low temperature and high hydrostatic pressure. Appl. Environ. Microbiol. 2002;68:456–463
  150. Wernersson, R., Schierup, M.H., Jørgensen. F.G., et al., 2005. Pigs in sequence space: a 0.66X coverage pig genome survey based on shotgun sequencing. BMC Genomics 6, p. 70. doi: 10.1186/1471–21 64–6-70.
  151. Whitten WK. Culture of tubal mouse ova. Nature. 1956;177:96–97
  152. Whitten WK. Culture of tubal ova. Nature. 1957;179:1081–1082
  153. Wood SA, Allen ND, Rossant J, et al. Non-injection methods for the production of embryonic stem cell-embryo chimaeras. Nature. 1993;365:87–89
  154. Wrenzycki C, Herrmann D, Lucas-Hahn A, et al. Messenger RNA expression patterns in bovine embryos derived from in vitro procedures and their implications for development. Reprod., Fertil. Develop. 2005;17:23–35
  155. Yoshioka K, Suzuki C, Tanaka A, et al. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 2000;66:112–119
  156. Yovich J, Grudzinskas G. The Management of Infertility: A Manual of Gamete Handling Procedures. Oxford: Heinemann Medical Books; 1990;
  157. Zander DL, Thompson JG, Lane M. Perturbations in mouse embryo development and viability caused by ammonium are more severe after exposure at the cleavage stages. Biol. Reprod. 2006;74:288–294

 Gábor Vajta obtained an MD degree (1976), a speciality (1979) and a PhD degree in human pathology (1988) in Hungary, and a Doctor of Veterinary Sciences degree (1999) in Denmark. He is adjunct professor at the University of Copenhagen, Denmark and James Cook University, Australia. He is author of more than 100 publications, and member of the Editorial Board of Cloning and Stem Cells.

PII: S1472-6483(09)00302-2

doi: 10.1016/j.rbmo.2009.12.018

Reproductive BioMedicine Online
Volume 20, Issue 4 , Pages 453-469 , April 2010

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