Human ovarian tissue cryopreservation: quality of follicles as a criteria of effectiveness
Received 30 July 2009; received in revised form 24 September 2009; accepted 9 December 2009. published online 12 February 2010.
Abstract
Experiments comparing vitrification and conventional freezing of mammalian ovarian tissue show that vitrification can also guarantee the storage of viable follicles after warming, but conventional freezing is more effective. The central goal of cryotechnology is the preservation of intact follicles. This article presents a critical opinion about the normality of follicles after vitrification of human ovarian tissue and microbial contamination as a result of direct contact of this tissue with liquid nitrogen at vitrification.
Declaration: The authors report no financial or commercial conflicts of interest.
ABSTRACT