Reproductive BioMedicine Online
Volume 21, Issue 3 , Pages 274-277 , September 2010

Embryo aneuploidy and the role of morphological and genetic screening

Received 15 June 2010 ,Accepted 23 June 2010.

References 

  1. Alfarawati, S., Fragouli, E., Colls, P., et al., 2010. The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender. Fertil. Steril., in press, doi:10.1016/j.fertnstert.2010.04.003.
  2. Baart EB, Van Opstal D, Los FJ, Fauser BC, Martini E. Fluorescence in-situ hybridization analysis of two blastomeres from day-3 frozen-thawed embryos followed by analysis of the remaining embryo on day-5. Hum. Reprod. 2004;19:685–693
  3. Braude P, Bolton V, Moore S. Human gene expression first occurs between the four- and eight-cell stages of preimplantation development. Nature. 1988;332:459–461
  4. Cohen J, Wells D, Munné S. Removal of two cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests employed to enhance implantation rates. Fertil. Steril. 2007;87:496–503
  5. Colls P, Escudero T, Cekleniak N, Sadowy S, Cohen J, Munné S. Increased efficiency of preimplantation genetic diagnosis for infertility using ‘no result rescue’. Fertil. Steril. 2007;88:53–61
  6. Coulam CB, Jeyendram RS, Fiddler M, Pergament E. Discordance among blastomeres renders preimplantation genetic diagnosis for aneuploidy ineffective. J. Assist. Reprod. Genet. 2007;24:37–41
  7. De Vos A, Staessen C, De Rycke M, et al. Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers. Hum. Reprod. 2009;12:2988–2996
  8. Eaton JL, Hacker MR, Harris D, Thornton KL, Penzias AS. Assessment of day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage. Fertil. Steril. 2009;91:2432–2436
  9. Finn, A., Scott, L., Davies, D., Hill, J., in press. Sequential embryo scoring as a predictor of aneuploidy in poor-prognosis patients. Reprod. Biomed. Online, doi:10.1016/j.rbmo.2010.05.004.
  10. Fragouli E, Wells D, Thornhill A, et al. Comparative genomic hybridization analysis of human oocytes and polar bodies. Hum. Reprod. 2006;21:2319–2328
  11. Fragouli E, Katz-Jaffe M, Alfarawati S, et al. Comprehensive chromosome screening of polar bodies and blastocysts from couples experiencing repeated implantation failure. Fertil. Steril. 2009;(Epub ahead of print)
  12. Fragouli E, Bianchi V, Patrizio , et al. Transcriptomic profiling of human oocytes: association of meiotic aneuploidy and altered oocyte gene expression. Mol. Hum. Reprod. 2010;(Epub ahead of print)
  13. Gianaroli L, Magli MC, Ferraretti AP, et al. The beneficial effects of PGD for aneuploidy support extensive clinical application. Reprod. BioMed. Online. 2004;10:633–640
  14. Hardarson T, Caisander G, Sjögren A, Hanson C, Hamberger L, Lundin K. A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts. Hum. Reprod. 2003;18:399–407
  15. Hardarson T, Hanson C, Lundin K, et al. Preimplantation genetic screening in women of advanced maternal age caused a decrease in clinical pregnancy rate: a randomized controlled trial. Hum. Reprod. 2008;23:2806–2812
  16. Kuliev A, Cieslak J, Ilkevitch Y, Verlinsky Y. Chromosomal abnormalities in a series of 6, 733 human oocytes in preimplantation diagnosis for age-related aneuploidies. Reprod. Biomed. Online. 2003;6:54–59
  17. Magli MC, Gianaroli L, Ferraretti AP, Lappi M, Ruberti A, Farfalli V. Embryo morphology and development are dependent on the chromosome complement. Fertil. Steril. 2007;87:534–541
  18. Mastenbroek S, Twisk M, van Echten-Arends J, et al. Preimplantation genetic screening in women of advanced maternal age. New Engl. J. Med. 2007;357:9–17
  19. Moayeri SE, Allen RB, Brewster WR, Kim MH, Porto M, Werlin LB. Day-3 embryo morphology predicts euploidy among older subjects. Fertil. Steril. 2008;89:118–123
  20. Munné S, Lee A, Rosenwaks Z, Grifo J, Cohen J. Diagnosis of major chromosome aneuploidies in human preimplantation embryos. Hum. Reprod. 1993;8:2185–2191
  21. Munné S, Sandalinas M, Escudero T, et al. Improved implantation after preimplantation genetic diagnosis of aneuploidy. Reprod. Biomed. Online. 2003;7:91–97
  22. Munné S, Chen S, Colls P, et al. Maternal age, morphology, development and chromosome abnormalities in over 6000 cleavage-stage embryos. Reprod. Biomed. Online. 2007;14:628–634
  23. Munné S, Howles CM, Wells D. The role of preimplantation genetic diagnosis in diagnosing embryo aneuploidy. Curr. Opin. Obstet. Gynecol. 2009;21:442–449
  24. Munné, S., Wells, D., Cohen, J., in press. Technology requirements for preimplantation genetic diagnosis to improve art outcome. Fertil. Steril.
  25. Pellestor F, Andréo B, Arnal F, Humeau C, Demaille J. Maternal aging and chromosomal abnormalities: new data drawn from in-vitro unfertilized human oocytes. Hum. Genet. 2003;112:195–203
  26. Sandalinas M, Marquez C, Munne S. Spectral karyotyping of fresh, noninseminated oocytes. Mol. Hum. Reprod. 2002;8:580–585
  27. Schoolcraft WB, Fragouli E, Stevens J, Munne S, Katz-Jaffe MG, Wells D. Clinical application of comprehensive chromosomal screening at the blastocyst stage. Fertil. Steril. 2009;(Epub ahead of print)
  28. Staessen C, Van Steirteghem AC. The genetic constitution of multinucleated blastomeres and their derivative daughter blastomeres. Hum. Reprod. 1998;13:1625–1631
  29. Staessen C, Platteau P, Van Assche E, et al. Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial. Hum. Reprod. 2004;19:2849–2858

PII: S1472-6483(10)00444-X

doi: 10.1016/j.rbmo.2010.06.035

Reproductive BioMedicine Online
Volume 21, Issue 3 , Pages 274-277 , September 2010

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