Reproductive BioMedicine Online
Volume 21, Issue 6 , Pages 762-768 , December 2010

Group culture of human zygotes is superior to individual culture in terms of blastulation, implantation and life birth

  • T. Ebner

      Affiliations

    • Landes- Frauen- und Kinderklinik, IVF-Unit, Linz, Upper Austria, Austria
    • Corresponding Author InformationCorresponding author.
  • ,
  • O. Shebl

      Affiliations

    • Landes- Frauen- und Kinderklinik, IVF-Unit, Linz, Upper Austria, Austria
  • ,
  • M. Moser

      Affiliations

    • Landes- Frauen- und Kinderklinik, IVF-Unit, Linz, Upper Austria, Austria
  • ,
  • R.B. Mayer

      Affiliations

    • Landes- Frauen- und Kinderklinik, IVF-Unit, Linz, Upper Austria, Austria
  • ,
  • W. Arzt

      Affiliations

    • Landes- Frauen- und Kinderklinik, Institute of Prenatal Genetics, Upper Austria, Austria
  • ,
  • G. Tews

      Affiliations

    • Landes- Frauen- und Kinderklinik, IVF-Unit, Linz, Upper Austria, Austria

Received 27 January 2010 ,Revised 26 May 2010 ,Accepted 24 June 2010.

References 

  1. Almagor M, Bejar C, Kafka I, et al. Pregnancy rates after communal growth of preimplantation human embryos in vitro. Fertil. Steril. 1996;66:394–397
  2. Biggers JD, Summers MC. Choosing a culture medium: making informed choices. Fertil. Steril. 2008;90:473–483
  3. Bolton VN, Wren ME, Parsons JH. Pregnancies after in vitro fertilization and transfer of human blastocysts. Fertil. Steril. 1991;55:830–832
  4. Ebner T, Yaman C, Moser M, et al. A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features. J. Assist. Reprod. Genet. 2001;18:601–606
  5. Ebner T, Moser M, Sommergruber M, et al. Presence, but not type or degree of extension, of a cytoplasmic halo has a significant influence on preimplantation development and implantation behaviour. Hum. Reprod. 2003;18:2406–2412
  6. Ebner T, Moser M, Sommergruber M, et al. Incomplete denudation of oocytes prior to ICSI enhances embryo quality and blastocyst development. Hum. Reprod. 2006;21:2972–2977
  7. Ebner T, Moser M, Shebl O, et al. Morphological analysis at compacting stage is a valuable prognostic tool for ICSI patients. Reprod. Biomed. Online. 2009;18:61–66
  8. Ebner T, Vanderzwalmen P, Shebl O, et al. Morphology of vitrified/warmed day-5 embryos predicts rates of implantation, pregnancy and live birth. Reprod. Biomed. Online. 2009;19:72–78
  9. Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF?. Hum. Reprod. Update. 1997;3:367–382
  10. Gardner DK, Lane MW, Lane M. Development of the inner cell mass in mouse blastocysts is stimulated by reducing the embryo:incubation volume ratio. Hum. Reprod. 1997;12(Abstract book):132
  11. Gardner DK, Lane M, Stevens J, et al. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil. Steril. 2000;73:1155–1158
  12. Gopichandran N, Leese HJ. The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos. Reproduction. 2006;131:269–277
  13. Goto Y, Kanzaki H, Nakayama T, et al. Relationship between the day of embryo transfer and the outcome in human in vitro fertilization and embryo transfer. J. Assist. Reprod. Genet. 1994;11:401–404
  14. Hardy K, Handyside AH, Winston RM. The human blastocyst: cell number, death and allocation during late preimplantation development in vitro. Development. 1989;107:597–604
  15. Lane M, Gardner DK. Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro. Hum. Reprod. 1992;7:558–562
  16. Lane M, Gardner DK. Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse. Biol. Reprod. 2003;69:1109–1117
  17. Lu DP, Chandrakanthan V, Cahana A, et al. Trophic signals acting via phosphatidylinositol-3-kinase are required for normal pre-implantation mouse embryo development. J. Cell Sci. 2003;117:1567–1576
  18. Moessner J, Dodson WC. The quality of human embryo growth is improved when embryos are cultured in groups rather than separately. Fertil. Steril. 1995;64:1034–1035
  19. Montag M, Van der Ven H. Evaluation of pronuclear morphology as the only selection criterion for further embryo culture and transfer: results of a prospective multicentre study. Hum. Reprod. 2001;16:2384–2389
  20. O’Doherty EM, Wade MG, Hill JL, et al. Effects of culturing bovine oocytes either singly or in groups on development to blastocysts. Theriogenology. 1997;48:161–169
  21. O’Neill C. Evidence for the requirement of autocrine growth factors for development of mouse preimplantation embryos in vitro. Biol. Reprod. 1997;56:229–237
  22. O’Neill C. Role of autocrine mediators in the regulation of embryo viability: lessons from animal models. J. Assist. Reprod. Genet. 1998;15:460–465
  23. O’Neill C. The potential roles for embryotrophic ligands in preimplantation embryo development. Hum. Reprod. Update. 2008;14:275–288
  24. Orsi NM, Leese HJ. Ammonium exposure and pyrovate affect the amino acid metabolism of bovine blastocysts in vitro. Reproduction. 2004;127:131–140
  25. Paria BC, Dey SK. Preimplantation embryo development in vitro: co-operative interactions among embryos and role of growth factors. Proc. Natl. Acad. Sci. USA. 1990;87:4756–4760
  26. Rijnders PM, Jansen CA. Influence of group culture and culture volume on the formation of human blastocysts: a prospective randomized study. Hum. Reprod. 1999;14:2333–2337
  27. Roberts C, O’Neill C, Wright L. Platelet activating factor (PAF) enhances mitosis in preimplantation mouse embryos. Reprod. Fertil. Dev. 1993;5:271–279
  28. Roudebush WE, Purnell ET, Stoddart NR, et al. Embryonic platelet-activating factor: temporal expression of the ligand and receptor. J. Assist. Reprod. Genet. 2002;19:72–78
  29. Roussev RG, Coulam CB, Kaider BD, et al. Embryonic origin of preimplantation factor (PIF): biological activity and partial characterization. Mol. Hum. Reprod. 1996;2:883–887
  30. Salahuddin S, Ookutsu S, Goto K, et al. Effects of embryo density and co-culture of unfertilized oocytes on embryonic development of in-vitro fertilized mouse embryos. Hum. Reprod. 1995;10:2382–2385
  31. Spanos S, Becker DL, Winston RML, et al. Anti-apoptotic action of insulin-like growth factor-I during human preimplantation embryo development. Biol. Reprod. 2000;63:1413–1420
  32. Spyropoulou I, Karamalegos C, Bolton VN. A prospective randomized study comparing the outcome of in-vitro fertilization and embryo transfer following culture of human embryos individually or in groups before embryo transfer on day 2. Hum. Reprod. 1999;14:76–79
  33. Stojanovic T, Alechna S, O’Neill C. In-vitro fertilization and culture of mouse embryos in vitro significantly retards the onset of insulin-like growth factor-II expression from the zygotic genome. Mol. Hum. Reprod. 1999;5:116–124
  34. Teruel M, Smith R. Effect of embryo density and growth factors on in vitro preimplantation development of mouse embryos. Acta Physiol. Pharmacol. Ther. Latinoam. 1997;47:87–96
  35. Teruel MT, Catalano RC, Cabodevila JA, et al. Regulation of mouse embryo development by autocrine trophic factors. Biocell. 2005;29:183–186
  36. Virant-Klun I, Tomazevic T, Vrtazcnik-Bokal E, et al. Increased ammonium in culture medium reduces the development of human embryos to the blastocyst stage. Fertil. Steril. 2006;85:526–528
  37. Wiley LM, Yamami S, Van Muyden D. Effect of potassium concentration, type of protein supplement, and embryo density on mouse preimplantation development in vitro. Fertil. Steril. 1986;45:111–119

 Thomas Ebner, PhD, graduated with honours from the Paris Lodron University of Salzburg, Austria, in 1992. Completing his doctorate and post-doctoral thesis, he became a university lecturer in Salzburg. He has published more than 80 papers as first and co-author. Research interests include non-invasive IVF selection processes, laser application, vitrification and culture media. He was certified as a senior clinical embryologist in 2008. Currently he is scientific director of the European School of ART in Linz.

PII: S1472-6483(10)00450-5

doi: 10.1016/j.rbmo.2010.06.038

Reproductive BioMedicine Online
Volume 21, Issue 6 , Pages 762-768 , December 2010