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Overnight ovarian tissue transportation for centralized cryobanking: a feasible option

Published:January 18, 2019DOI:https://doi.org/10.1016/j.rbmo.2019.01.006

      Abstract

      Research question

      Is overnight transportation of ovarian tissue before cryopreservation in a centralized cryobank from the FertiPROTEKT network feasible?

      Design

      Data from 1810 women with cryopreserved ovarian tissue after overnight transportation from December 2000 to December 2017 were analysed with a focus on transportation, tissue activity parameters and pregnancy, and delivery rates after transplantation.

      Results

      A total of 92.4% of tissue samples arrived at ideal temperatures of 2–8°C, 0.4% were transported at temperatures lower than ideal and 6.4% were transported at temperatures that were too high, generally due to mishandling of the inlayed cool packs of the transportation boxes. In 62 women, 78 tissue transplantations were carried out. A subgroup of 30 women who underwent a single orthotopic transplantation with fulfilled criteria of a complete follow-up after transplantation until the end of study, a premature ovarian insufficiency after gonadotoxic therapy as well as the absence of pelvic radiation, was further analysed. In this group, transplantations into a peritoneal pocket accounted for 90%. Transplants were still active at 1 year and above after transplantation in 93.3%. Pregnancy and delivery rates were 46.7% and 43.3%, respectively, with one ongoing pregnancy at the end of the study.

      Conclusions

      Overnight transportation for central cryobanking is a feasible concept that results in high reproducible success rates through standardized professional tissue freezing and storage.

      KEYWORDS

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      Biography

      Jana Liebenthron obtained her PhD at the University Clinic Bonn under the supervision of Professor Markus Montag. In 2014, she assumed the position of Director of the IVF-Lab and Cryobank for human gametes and ovarian tissue. She is an active executive board member of the network FertiProtekt, highly specialized for ovarian tissue-banking and supports national and international hospitals in this field of fertility preservation. She has managed the new department UniCareD at the University clinic Düsseldorf since January 2018.
      Key message
      Ovarian tissue cryopreservation is gaining more acceptance in clinical practice as an established option for fertility protection. Nevertheless, as the techniques require high expertise for reproducible success rates, the concept of centralized cryobanking can be carried over by fertility preservation networks to allow optimal tissue freezing and storage for everyone.