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miR-532-3p: a possible altered miRNA in cumulus cells of infertile women with advanced endometriosis

  • Liliane Fabio Isidoro da Silva
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil
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  • Michele Gomes Da Broi
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil

    National Institute of Hormones and Women's Health – CNPq, Porto Alegre, Brazil
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  • Caroline Mantovani da Luz
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil
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  • Lilian Eslaine Costa Mendes da Silva
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil
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  • Rui Alberto Ferriani
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil

    National Institute of Hormones and Women's Health – CNPq, Porto Alegre, Brazil
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  • Juliana Meola
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil

    National Institute of Hormones and Women's Health – CNPq, Porto Alegre, Brazil
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  • Paula Andrea Navarro
    Correspondence
    Corresponding author.
    Affiliations
    Division of Human Reproduction, Department of Gynecology and Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto – USP, São Paulo, Brazil

    National Institute of Hormones and Women's Health – CNPq, Porto Alegre, Brazil
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Published:October 22, 2020DOI:https://doi.org/10.1016/j.rbmo.2020.10.010

      Abstract

      Research question

      Is the profile of microRNA (miRNA) altered in cumulus cells of infertile women with early (EI/II) and advanced (EIII/IV) endometriosis?

      Design

      In this prospective case–control study, a miRNA profile including 754 targets was evaluated in samples of cumulus cells from infertile women with endometriosis (5 EI/II, 5 EIII/IV) and infertile controls (5, male and/or tubal factor) undergoing ovarian stimulation for intracytoplasmic sperm injection, using TaqMan® Array Human MicroRNA Cards A and B. The groups were compared with Kruskal–Wallis test, followed by Benjamini–Hochberg correction and Dunn's post hoc test. An in silico enrichment analysis was performed to list the possibly altered pathways in which the altered miRNA target genes are involved.

      Results

      Only the miRNA miR-532-3p showed significant differences among the analysed groups, being down-regulated in the EIII/IV group compared with the infertile control group, as well as compared with the EI/II group. The enrichment analysis showed that some genes regulated by this miRNA are involved in important pathways for the acquisition of oocyte competence, such as the oxytocin, calcium, Wnt, FoxO, ErbB and Ras signalling pathways, as well as the oocyte meiosis pathway.

      Conclusion

      The present findings bring new perspectives to understanding the follicular microenvironment of infertile women with different stages of endometriosis. It is suggested that the dysregulation of miR-532-3p may be a potential mechanism involved in the aetiopathogenesis of endometriosis-related infertility. Further studies are needed to evaluate these pathways in cumulus cells of infertile women with the disease, as well as their impact on the acquisition of oocyte competence.

      Keywords

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      Biography

      Paula A Navarro (MD, PhD) is Associate Professor, Division of Human Reproduction, Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto – USP and Director of the Assisted Reproduction Laboratory. She has conducted research on infertility and assisted reproduction, trying to understand the aetiopathogenic mechanisms of infertility related to endometriosis and perfecting fertility preservation strategies.
      Key message
      A miRNA profile was evaluated in cumulus cells from infertile women with early (EI/II) and advanced (EIII/IV) endometriosis and controls undergoing ovarian stimulation for intracytoplasmic sperm injection. Only miR-532-3p was down-regulated in the EIII/IV group compared to infertile controls and EI/II groups. It is suggested that dysregulation of miR-532-3p in cumulus cells is a potential mechanism in the aetiopathogenesis of advanced endometriosis-related infertility.