Comparison of live birth rates after IVF–embryo transfer with and without preimplantation genetic testing for aneuploidies

Published:September 20, 2021DOI:


      Research question

      Does the use of preimplantation genetic testing for aneuploidies (PGT-A) result in higher live birth rates when compared with both fresh and frozen embryo transfers (FET) not utilizing PGT-A?


      Retrospective cohort study at a single tertiary centre using inverse probability of treatment weighting (IPTW) to adjust for differences in baseline characteristics between groups.


      A total of 107 FET using PGT-A from 74 patients, along with 321 fresh and 286 FET not using PGT-A from 381 patients met the inclusion criteria for this study. In the IPTW-adjusted analysis of transfer-level data, PGT-A transfers resulted in a significantly higher live birth rate when compared with both non-PGT-A fresh (49.5% versus 38.6%, P = 0.036) and FET (50.6% versus 35.8%, P = 0.016). When data were analysed per retrieval level, the live birth rate was similar and acceptably high with or without PGT-A (63.7% versus 52.3%, P = 0.09).


      When comparing PGT-A to non-PGT-A fresh and FET, PGT-A embryo transfers have a significantly higher live birth rate. However, this difference did not persist at a per-retrieval level. Further investigation is needed to understand in what scenarios PGT-A has clinical significance and whether differences in the number of available embryos for transfer negates the benefit of PGT-A.


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      Emily Sadecki is a fourth year medical student at Mayo Clinic Alix School of Medicine and recent graduate of the Master of Bioethics programme at the University of Pennsylvania.
      Key message
      Although live birth rates are increased with use of preimplantation genetic testing for aneuploidies (PGT-A) compared with non-PGT-A at a transfer level, the differences are no longer statistically significant at a retrieval-level. Further studies are needed to establish which patient groups would benefit from the use of PGT-A.