A newly developed carrier for the vitrification of prepubertal testicular tissue and its comparison with four different carriers

Published:December 07, 2021DOI:


      Research Question

      : The cryopreservation of prepubertal testicular tissue is important for children who will undergo gonadotoxic treatment. The debate on the optimal carrier for an inexpensive and rapid vitrification technique is ongoing. The aim of this study was to evaluate the efficiency of a novel, practical and sterilisable metal brush and to compare it with previously used carriers.


      : The testicular tissues of prepubertal rats were vitrified using four different carriers and evaluated by light microscopy and transmission electron microscopy.


      : Nuclei were easily discriminated in the metal brush, aluminium foil, and high-security straw groups, but the discrimination of structures decreased in the metal wire group. Minimal cytoplasmic degeneration, vacuolisation and mild reversible degenerative effects were seen in spermatogonial stem cells and Sertoli cells in the metal brush group. Mild to moderate structural changes were found in the aluminium foil group. Severe pyknosis of the nuclei, a high degree of swelling, expansion of the endoplasmic reticulum and swelling and blurring of the mitochondria were seen in the metal wire and high-security straw groups. The cell viabilities in the metal brush, aluminium foil, metal wire, and high-security straw groups were 91.6 ± 3.8%, 83.0 ± 4.0%, 76.0 ± 3.1% and 68.6 ± 4.9%, respectively.


      : The metal brush is a promising new carrier for prepubertal testis vitrification.


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      Assist. Prof. Kervancioglu, M.D., Ph.D., is the head of the Histology and Embryology Department at Istanbul Aydın University. She worked as the senior scientist at a government andrology laboratory for 17 years. Her research focus is fertility preservation and male infertility.
      Key message
      • The debate on the optimal carrier for vitrification of prepubertal testicular tissue is ongoing. • We developed a practical and sterilisable carrier, the metal brush, and compared it with others. • The results suggest that the metal brush is good at preserving tissue morphology and is a promising carrier.