Advertisement

Optimal puncture position for ICSI can be detected by image analysis using Local Binary Pattern

  • Takashi Morimoto
    Correspondence
    Corresponding author.
    Affiliations
    Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan
    Search for articles by this author
  • Tomohiro Maekawa
    Affiliations
    Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan
    Search for articles by this author
  • Shimpei Mizuta
    Affiliations
    Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan

    Department of Reproductive Medicine, Reproduction Clinic Osaka, Grand Front Osaka Tower A 15F, 4-20 Ofuka-cho, Kita-ku, Osaka 530-0011, Japan
    Search for articles by this author
  • Hidehiko Matsubayashi
    Affiliations
    Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan

    Department of Reproductive Medicine, Reproduction Clinic Osaka, Grand Front Osaka Tower A 15F, 4-20 Ofuka-cho, Kita-ku, Osaka 530-0011, Japan
    Search for articles by this author
  • Takumi Takeuchi
    Affiliations
    Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan
    Search for articles by this author
  • Yutaka Hata
    Affiliations
    Graduate School of Simulation Studies, University of Hyogo, 7-1-28, Minatojimaminami-cho, Chuo-ku, Kobe, Japan
    Search for articles by this author
  • Tomomoto Ishikawa
    Affiliations
    Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan

    Department of Reproductive Medicine, Reproduction Clinic Osaka, Grand Front Osaka Tower A 15F, 4-20 Ofuka-cho, Kita-ku, Osaka 530-0011, Japan
    Search for articles by this author
Published:September 18, 2022DOI:https://doi.org/10.1016/j.rbmo.2022.09.011

      Abstract

      Research question

      One of the problems during the intracytoplasmic sperm injection (ICSI) procedure is unintentional membrane rupture (UMR), which often predisposes to subsequent oocyte degeneration. Can the ICSI Position Detector (IPD) be useful in identifying the optimal puncture location to prevent UMR during ICSI?

      Design

      A total of 709 mature oocytes were included. Conventional ICSI was carried out and images were recorded by IPD; these were analysed retrospectively.

      Results

      Inseminated oocytes were retrospectively grouped according to the IPD, irrespective of whether oolemma was punctured at an area in which UMR is likely (non-appropriate group) or unlikely (appropriate group). In the appropriate group, rates of UMR (5.3% versus 18.2%) and degeneration (2.5% versus 8.7%) were significantly lower than those of the non-appropriate group, whereas rate of fertilization (87.1% versus 69.7%) was significantly higher than those of the non-appropriate group, respectively (P < 0.001). These differences remained even after propensity score matching to adjust for potential differences in characteristics between appropriate and non-appropriate groups.

      Conclusions

      This study demonstrated that the IPD is useful to identify the optimal puncture location to circumvent UMR during the ICSI procedure, resulting in reduced UMR and oocyte degeneration, thereby, generating more embryos available for transfer or cryopreservation.

      KEYWORDS

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Reproductive BioMedicine Online
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Abdelmassih S.
        • Cardoso J.
        • Abdelmassih V.
        • Dias A.J.
        • Abdelmassih R.
        • Nagy P.Z.
        Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates.
        Hum. Reprod. 2002; 17: 2694-2699
      1. Adamson, D., Zegers, F., Mouzon, D.J., Ishihara, O., Dyer, S., Banker, M., Chambers, G., Kupka Markus. ICMART World Collaborative Reports for assisted reproductive technology (ART) 2017; Available at:https://www.icmartivf.org/reports-publications/. Accessed June 26, 2021.

        • Ahonen T.
        • Hadid A.
        • Pietikäinen M.
        Face description with local binary patterns: application to face recognition.
        IEEE Transactions on Pattern Analysis and Machine Intelligence. 2006; 28: 2037-2041
        • Ahonen T.
        • Rahtu E.
        • Ojansivu V.
        • Heikkilä J.
        Recognition of blurred faces using local phase quantization.
        in: Proceedings of the 19th International Conference on Pattern Recognition (ICPR 2008) Tampa, FL. 2008: 4
        • Andersen N.A.
        • Carlsen E.
        • Loft A.
        Trends in the use of intracytoplasmatic sperm injection marked variability between countries.
        Hum. Reprod. Update. 2008; 14: 593-604
        • Babayev N.S.
        • Park W.C.
        • Bukulmez O.
        Intracytoplasmic sperm injection indications: how rigorous?.
        Semin. Reprod. Med. 2014; 32: 283-290
        • Demirol A.
        • Benkhalifa M.
        • Sari T.
        • Gurgan T.
        Use of laser-assisted intracytoplasmic sperm injection (ICSI) in patients with a history of poor ICSI outcome and limited metaphase II oocytes.
        Fertil. Steril. 2006; 86: 256-258
        • Fulka J.
        • Kárníková L.
        • Moor M.R.
        Oocyte polarity: ICSI, cloning and related techniques.
        Hum. Reprod. 1998; 13: 3303-3305
        • Gardner K.D.
        • Lane M.
        • Stevens J.
        • Schlenker T.
        • Schoolcraft B.W.
        Blastocyst score affects implantation and pregnancy outcome: Towards a single blastocyst transfer.
        Fertil. Steril. 2000; 73: 1155-1158
        • Hiraoka K.
        • Kitamura S.
        Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm.
        J. Assist. Reprod. Genet. 2015; 32: 1827-1833
        • Iwayama H.
        • Hochi S.
        • Yamashita M.
        Low stretching ability of human oolemma during piezo-ICSI as a risk factor in post-injection survival and implantation.
        J. Mamm. Ova. Res. 2010; 27: 150-156
        • Katagiri Y.
        • Jwa S.C.
        • Kuwahara A.
        • Iwasa T.
        • Ono M.
        • Kato K.
        • Kishi H.
        • Kuwabara Y.
        • Harada M.
        • Hamatani T.
        • Osuga Y.
        Assisted reproductive technology in Japan: A summary report for 2019 by the Ethics Committee of the Japan Society of Obstetrics and Gynecology.
        Reproductive Medicine and Biology. 2021; 21
        • Kimura Y.
        Development of Piezo ICSI.
        J. Mamm. Ova. Res. 2009; 26 (in Japanese): 69-78
        • Kinishi Y.
        • Maekawa T.
        • Mizuta S.
        • Ishikawa T.
        • Hata Y.
        Detection of optimal puncture position in ovum images for artificial insemination.
        in: Proceedings of the 2019 International Conference on Machine Learning and Cybernetics. 2019: 140-144
        • Mitchell P.R.
        • Shehua S.
        • Anthony T.D.
        • Victor Y.F.
        • Charles E.M.
        • Marcelle I.C.
        Oocyte degeneration after intracytoplasmic sperm injection: a multivariate analysis to assess its importance as a laboratory or clinical marker.
        Fertil. Steril. 2006; 85: 1736-1743
        • Mori K.
        • Kitaya K.
        • Ishikawa T.
        • Hata Y.
        A pregnancy prediction system based on uterine peristalsis from ultrasonic images.
        Intell. Autom. Soft Comput. 2021; 29: 335-352
        • Nagy P.Z.
        • Liu J.
        • Joris G.
        • Bocken G.
        • Desmet B.
        • Van Ranst A.
        • Vankelecom A.
        • Devroey P.
        • Van Steirteghem C.A.
        The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates.
        Hum. Reprod. 1995; 10: 3171-3177
        • Nishihara R.
        • Matsubayashi H.
        • Ishikawa T.
        • Mori K.
        • Hata Y.
        On Measurement System for Frequency of Uterine Peristalsis.
        IEICE Trans INF Syst. 2021; E104-D, No. 8: 1-7
        • Namgoong S.
        • Kim N.H.
        Roles of actin binding proteins in mammalian oocyte maturation and beyond.
        Cell Cycle. 2016; 15: 1830-1843
        • Ojala T.
        • Pietikäinen M.
        • Harwood D.
        Performance evaluation of texture measures with classification based on Kullback discrimination of distributions.
        in: Proceedings of the 12th International Conference on Pattern Recognition;, Jerusalem, Israel1994: 582-585
        • Ojala T.
        • Pietikäinen M.
        • Harwood D.
        A comparative study of texture measures with classification based on feature distributions.
        Pattern Recognition. 1996; 29: 51-59
        • Okishio K.
        • Koya Y.
        • Matsubayashi H.
        • Ishikawa T.
        Identifying an egg-containing follicle by displacement analysis of the follicle border.
        Transactions of the Institute of Systems, Control and Information Engineers. 2021; 34: 303-309
        • Palermo G.
        • Joris H.
        • Devroey P.
        • Van Steirteghem A.C.
        Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte.
        Lancet. 1992; 340: 17-18
        • Palermo D.G.
        • Alikani M.
        • Bertoli M.
        • Colombero T.L.
        • Moy F.
        • Cohen J.
        • Rosenwaks Z.
        Oolemma characteristics in relation to survival and fertilization patterns of oocytes treated by intracytoplasmic sperm injection.
        Hum. Reprod. 1996; 11: 172-176
        • Rienzi L.
        • Greco E.
        • Ubaldi F.
        • lacobelli M.
        • Martinez F.
        • Tesarik J.
        Laser-assisted intracytoplasmic sperm injection.
        Fertil. Steril. 2001; 76: 1045-1047
        • Stoddart N.R.
        • Fleming S.D.
        Orientation of the first polar body of the oocyte at 6 or 12 o'clock during ICSI does not affect clinical outcome.
        Hum. Reprod. 2000; 15: 1580-1585
        • Takeda T.
        Membrane Dynamics during Cytokinesis.
        BPPB. 2016; 56 (in Japanese): 13-17
        • Zhao G.
        • Pietikäinen M.
        Dynamic texture recognition using local binary patterns with an application to facial expressions.
        IEEE Transactions on Pattern Analysis and Machine Intelligence. 2007; 29: 915-928

      Biography

      Takashi Morimoto completed his BSC at Suzuka University of Medical Science, Japan, in 2016. He is currently an embryologist and Deputy Chief of Reproduction Clinic Tokyo, which has carried out over 4000 oocyte retrieval cycles and 3000 frozen embryo transfer cycles a year since 2017.
      Key message
      One of the complications during intracytoplasmic sperm injection is unintentional membrane rupture (UMR) and subsequent degeneration of the oocyte. Optimal puncture position for ICSI can be detected by image analysis using Local Binary Pattern, which may determine the location in which UMR is likely or unlikely to occur.