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Hsa_circ_0001550 impairs decidualization by regulating the proliferation and apoptosis of endometrial stromal cells

  • Author Footnotes
    1 Contributed equally.
    Meng Lyu
    Correspondence
    Corresponding authors.
    Footnotes
    1 Contributed equally.
    Affiliations
    The First Clinical Medical College of Lanzhou University, Lanzhou Gansu, China
    Search for articles by this author
  • Author Footnotes
    1 Contributed equally.
    Wenxin Gao
    Correspondence
    Corresponding authors.
    Footnotes
    1 Contributed equally.
    Affiliations
    The First Clinical Medical College of Lanzhou University, Lanzhou Gansu, China
    Search for articles by this author
  • Lili Zhang
    Affiliations
    The Reproductive Center, The First Hospital of Lanzhou University, Lanzhou Gansu, China

    Gansu Key Laboratory of Reproductive Medicine and Embryos, Lanzhou Gansu, China
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  • Xia Yang
    Affiliations
    The Reproductive Center, The First Hospital of Lanzhou University, Lanzhou Gansu, China

    Gansu Key Laboratory of Reproductive Medicine and Embryos, Lanzhou Gansu, China
    Search for articles by this author
  • Feng Yue
    Affiliations
    The Reproductive Center, The First Hospital of Lanzhou University, Lanzhou Gansu, China

    Gansu Key Laboratory of Reproductive Medicine and Embryos, Lanzhou Gansu, China
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  • Hongxing Li
    Affiliations
    The Reproductive Center, The First Hospital of Lanzhou University, Lanzhou Gansu, China

    Gansu Key Laboratory of Reproductive Medicine and Embryos, Lanzhou Gansu, China
    Search for articles by this author
  • Xiaoling Ma
    Correspondence
    Corresponding authors.
    Affiliations
    The Reproductive Center, The First Hospital of Lanzhou University, Lanzhou Gansu, China

    Gansu Key Laboratory of Reproductive Medicine and Embryos, Lanzhou Gansu, China
    Search for articles by this author
  • Lin Liu
    Correspondence
    Corresponding authors.
    Affiliations
    The First Clinical Medical College of Lanzhou University, Lanzhou Gansu, China

    The Reproductive Center, The First Hospital of Lanzhou University, Lanzhou Gansu, China

    Gansu Key Laboratory of Reproductive Medicine and Embryos, Lanzhou Gansu, China
    Search for articles by this author
  • Author Footnotes
    1 Contributed equally.
Published:October 10, 2022DOI:https://doi.org/10.1016/j.rbmo.2022.10.003

      Abstract

      Research question

      What is the molecular function of hsa_circ_0001550 in decidualization?

      Design

      Human endometrial stromal cells (HESC) were isolated from the endometrium tissues to build an in-vitro decidualization model. Different concentrations of medroxyprogesterone acetate (MPA) were used to observe whether the expression level of hsa_circ_0001550 was related to progesterone. Biological characteristics and distribution of hsa_circ_0001550 were determined by RNase R, actinomycin D (Act D) assay and cytoplasmic/nuclear fraction assay. Then the overexpression of hsa_circ_0001550 was achieved by adenovirus vector. Cell proliferation was determined by Cell Counting Kit-8 (CCK-8) assays. The cell cycle was assessed by flow cytometry analyses. Cell apoptosis was determined by annexin-V/propidium iodide double staining experiment and western blotting.

      Results

      The expression of hsa_circ_0001550 was decreased in decidua and decidualized HESC (P < 0.001, P = 0.014). Hsa_circ_0001550 is a covalently closed RNA molecule that was verified by RNase R assay and Act D assay (P = 0.012). Nuclear and cytoplasmic separation experiments confirmed that hsa_circ_0001550 was mainly distributed in the cytoplasm. Overexpression of hsa_circ_0001550 inhibited decidualization of HESC (P < 0.0001). Furthermore, overexpression of hsa_circ_0001550 inhibited proliferation by decreasing the number of S phase cells (P = 0.033). Annexin-V/propidium iodide double staining experiment and western blotting revealed that overexpression of hsa_circ_0001550 promoted HESC apoptosis (P < 0.001, P = 0.0139).

      Conclusions

      Hsa_circ_0001550 impairs decidualization of HESC. Progesterone decreases the expression of hsa_circ_0001550. The results may provide new insights into the cause of decidualization.

      KEYWORDS

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      Biography

      Lin Liu graduated from the University of Science and Technology of China, with a PhD in cell biology, majoring in reproductive biology. Her research interest is the pathogenesis and mechanism of recurrent implantation failure.
      Key message
      Hsa_circ_0001550 was determined to inhibit decidualization. Hsa_circ_0001550 inhibited the proliferation and cell cycle progression of human endometrial stromal cells, and promoted apoptosis. On the basis of these findings, the inhibition of hsa_circ_0001550 represents a direction for exploring new treatments for decidual defects.