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The efficacy of novel centrifugation-free sperm selection (Io-Lix) on sperm parameters and ICSI reproductive outcomes

Published:November 05, 2022DOI:https://doi.org/10.1016/j.rbmo.2022.11.002

      Abstract

      Research question

      What is the effect of a novel non-centrifugation method (Io-Lix) of sperm selection on sperm parameters and intracytoplasmic sperm injection (ICSI) reproductive outcomes?

      Design

      This pilot study elevated the capacity of the Io-Lix sperm selection protocol to improve sperm parameters (concentration, motility and sperm DNA fragmentation) of the neat ejaculate. Once established, the reproductive outcomes of Io-Lix selected spermatozoa were used for autologous and donor oocyte ICSI programmes and their efficacy compared with those using conventional swim-up.

      Results

      Io-Lix sperm selection resulted in lower sperm concentration yield (P < 0.001) and sperm DNA fragmentation (P < 0.001) but higher sperm motility (P < 0.001) when compared with spermatozoa in the neat ejaculate. When compared with swim-up sperm selection the Io-Lix protocol resulted in a 14.7% (P = 0.028) increase in pregnancy rate and 16.3% (P = 0.047) reduction in miscarriages in the autologous ICSI programme. A similar comparison of sperm selection procedures employed for a donor oocyte ICSI programme showed no difference in terms of their respective reproductive outcomes.

      Conclusions

      The Io-Lix sperm selection protocol resulted in improved pregnancy rate and reduction in miscarriage when applied to autologous ICSI, which was attributed to a reduction in the proportion of spermatozoa with DNA damage post-selection. A similar finding was not apparent in the donor oocyte programme, which may be associated with the capacity of the donor oocyte to repair sperm DNA post-syngamy.

      Keywords

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      Biography

      Yolanda Cabello is senior clinical embryologist and scientific lead at Overture Life, Madrid. For over 20 years, she has served as director of embryology, andrology and PGT laboratories of various assisted reproduction units. She has been Chief Investigator of five funded European projects, and co-author of 45 peer-reviewed journal articles and seven book chapters.
      Key message
      Io-Lix sperm selection resulted in an improved pregnancy rate when applied to autologous ICSI, which was attributed to a reduced proportion of spermatozoa with DNA damage. A similar finding was not apparent in a donor oocyte ICSI programme associated with the capacity of the oocyte to repair paternal DNA post-syngamy.