Abstract
Research question
What are the effects of platelet lysate on structure, function and epigenetic modifications
of heterotopically transplanted mouse ovarian tissues?
Design
Mice were divided into three groups (n = 17 per group): control (mice with no ovariectomy, grafting or treatment), autograft
and autograft plus platelet lysate (3 ml/kg at the graft sites). Inflammatory markers,
serum malondialdehyde (MDA) concentration and total antioxidant capacity were assessed
on day 7 after transplantation. Twenty-eight days after transplantation, stereological
and hormonal analyses were conducted. Chromatin immunoprecipitation and quantitative
real-time polymerase chain reaction were also used to quantify the epigenetic modifications
of maturation genes, parallel to their expression.
Results
The total volume of the ovary, cortex and medulla, and the number of different types
of follicles, the concentration of interleukin (IL)-10, progesterone and oestradiol
and total antioxidant capacity significantly decreased in the autograft group compared
with the control group (P < 0.001); these parameters significantly increased in the autograft plus platelet
lysate group compared with the autograft group (P < 0.001). The concentrations of tumour necrosis factor alpha, IL-6 and MDA increased
significantly in the autograft group compared with the control group (P < 0.001); in the autograft plus platelet lysate group, these parameters significantly
decreased compared with the autograft group (P < 0.001). In the autograft plus platelet lysate group, the expression levels of Gdf-9 (P < 0.0021), Igf-1 (P < 0.0048) and Igf-2 (P < 0.0063) genes also increased along with a lower incorporation of MeCP2 in the promoter
regions (P < 0.001) compared with the autograft group.
Conclusions
Platelet lysate can contribute to follicular survival by improving folliculogenesis
and increasing the expression of oocyte maturation genes.
KEYWORDS
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Biography
Malek Soleimani Mehranjani obtained his PhD at Sheffield University, UK. He returned to Arak University in Iran and jointly founded the Reproductive Biology Centre there. He is currently Professor at Arak University, and his major areas of interest are histological, pathological and clinical evaluation of the reproductive system.
Key message
Platelet lysate as a substrate for ovarian tissue grafting site can contribute to follicular survival and graft function restoration through reducing follicle loss induced by ischaemia reperfusion and reducing DNA methylation of the oocyte maturation genes, which ultimately increases the expression of these genes and enhances folliculogenesis.
Article info
Publication history
Published online: December 02, 2022
Accepted:
November 28,
2022
Received in revised form:
November 1,
2022
Received:
July 22,
2022
Declaration: The authors report no financial or commercial conflicts of interest.Identification
Copyright
© 2022 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
