Abstract
Research question
Is it feasible to apply conventional IVF to couples undergoing preimplantation genetic
testing for aneuploidies (PGT-A) with non-severe male infertility?
Design
The last wash fluid of biopsied trophectoderm (TE) cells was collected for whole genome
amplification (WGA). A method was developed to determine parental contamination. Using
single-nucleotide polymorphism (SNP) analysis, two standard curves were established;
further mixtures were used for verification. Finally, 29 WGA products from couples
undergoing conventional IVF were used to evaluate parental contamination.
Results
The WGA results of the last wash fluid of biopsied TE cells revealed almost no free
DNA. By adopting two strategies based on maternally and paternally biased SNP in the
mixture, data from bioinformatics analysis were analysed to determine the relationship
between maternal (Index M) and paternal (Index F) bias statistics. Two standard curves
were successfully established based on these indices that allowed the prediction of
maternal and parental contamination, which correlated well with actual ratios of known
composition mixtures during validation. The average contamination level was 10.6%
determined from 10 WGA products that featured maternal contamination, whereas that
of the other 19 products that featured paternal contamination was less than 10%.
Conclusions
This study confirmed the feasibility of applying conventional IVF to couples undergoing
PGT-A with non-severe male infertility.
Keywords
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Biography
Professor Yun Liu is Director of the Reproductive Medical Center, 900th Hospital of the Joint Logistics Team. She is a member of the National Expert Bank on Assisted Reproductive Technology Management. Professor Liu established the first reproductive medical centre with the qualification of PGT technology in Fujian Province in 2012.
Key message
Almost no free DNA was observed in the last wash fluid of biopsied TE cells. A method for detecting paternal contamination showed that amplified DNA samples of biopsied TE cells exhibited a low parental contamination rate. The feasibility of conventional IVF for couples undergoing PGT-A with non-severe male infertility was confirmed.
Article info
Publication history
Published online: December 20, 2022
Accepted:
December 14,
2022
Received in revised form:
November 23,
2022
Received:
August 17,
2022
Declaration: The authors report no financial or commercial conflicts of interest.Identification
Copyright
© 2022 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
