ABSTRACT
Research question
: What is the implication of aberrant CD24 expressions in endometriosis (EM) and the
relevant mechanisms?
Design
: Thirty women with EM and twenty-seven women with other benign gynecological diseases,
who underwent laparoscopy and hysteroscopy, were recruited in this study. The hEM15A
cells were used in functional experiments in vitro. The microarray assay and bioinformatics analyses were performed to investigate the
differentially expressed genes (DEGs) and signaling pathways regulated by CD24.
Results
: CD24 was expressed at significantly higher levels in eutopic endometrial (EU) tissues
than in paired ectopic endometrial (EC) tissues from EM patients and normal endometrial
(NE) tissues from controls. However, the CD24 expressions had no obvious relationships
with clinical features such as age, infertility conditions, and menstrual cycle phase.
Suppression of CD24 by small interfering RNAs in hEM15A cells impaired cell migration
and reduced cytoskeletal filamentous actin (F-actin), but had no effects on cell apoptosis,
cell cycle or cell viability. A total of 862 DEGs were identified between siCD24 and
siNC, which consisted of 517 upregulated genes and 345 downregulated genes. The gene
ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment
analyses for the DEGs mainly focused on the type I interferon signaling pathway and
TGF-beta signaling pathway. Gene set enrichment analysis (GSEA) was also conducted.
Furthermore, a protein-protein interaction (PPI) network and subnetwork were constructed
to explore the hub modules and DEGs.
Conclusions
: This study provides a better understanding of the role of CD24 in EM.
KEYWORDS
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Biography
Xiaohong Chang is Professor at Department of Obstetrics and Gynecology in Peking University People's Hospital. She has been working on the clinical and basic research into endometriosis and ovarian cancer for years.
Article info
Publication history
Accepted:
December 21,
2022
Received in revised form:
November 28,
2022
Received:
August 23,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2022 Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd.
