Highlights
- •Ovarian stimulation alone or in association with IVF promoted changes in phospholipid profile and abundance of mice blastocysts.
- •Oocyte vitrification promoted a reduced relative abundance of lipids in the blastocysts.
- •Blastocysts from oocytes vitrified in lipid-based media showed a less disturbed lipid profile.
ABSTRACT
Research question
Is the membrane lipid profile of mice blastocysts affected by ovarian stimulation
(S), in vitro fertilization (IVF), and oocyte vitrification? Could the supplementation
of vitrification media with L-carnitine (LC) and fatty acids (FA) prevent membrane
phospholipid changes in blastocysts from vitrified oocytes?
Design
Experimental study comparing the lipid profile of murine blastocysts produced from
natural mating (N), superovulated cycles (S), or after in vitro fertilization (IVF)
submitted or not to vitrification. For in vitro experiments, 562 oocytes from superovulated
females were randomly divided into 4 groups: fresh in vitro-fertilized oocytes (IVF)
and vitrified groups: Irvine Scientific (IRV); Tvitri-4 (T4) or Tvitri-4 supplemented
with LC and FA (T4-LC/FA). Fresh or vitrified/warmed oocytes were inseminated and
cultured for 96 or 120 hours. The lipid profile of the blastocysts from all experimental
groups was assessed by the MRM-profiling method.
Results
We profiled 125 lipids in blastocysts, and the statistical analysis revealed several
classes of phospholipids affected in the blastocysts by ovarian stimulation, IVF,
and/or oocyte vitrification. Regarding vitrification, results demonstrated that LC
and FA supplements prevented, to a certain extent, changes in phospholipid and sphingolipid
contents in comparison to IVF fresh oocytes.
Conclusion
Ovarian stimulation alone or in association with IVF promoted changes in phospholipid
profile and abundance of blastocysts. For the first time, we demonstrated that even
a short exposure time to the lipid-based solutions during oocyte vitrification was
sufficient to induce changes in the lipid profile that were sustained until the blastocyst
stage.
Key words
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Biography
Thalita Berteli is a Senior Embryologist. She holds a PhD in Reproductive Biology from the University of São Paulo (USP). She previously obtained her Master degree from the Department of Gynecology and Obstetrics (USP). Her research interests have focused on oocyte vitrification and the impact of IVF technologies on oocyte and embryo lipid profile.
Article info
Publication history
Accepted:
January 6,
2023
Received in revised form:
November 27,
2022
Received:
July 30,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2023 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
