Abstract
Research question
Does RNA binding protein with multiple splicing 2 (RBPMS2), a maternal factor that
accumulate and stored in the cytoplasm of mature oocytes, influenced early embryo
development in mice?
Design
The expression pattern of rbpms2 in mouse were analyzed by qRT-PCR and immunofluorescence
staining. The effect of knockdown of RBPMS2 on embryo development was evaluated through
microinjection of specific morpholino or siRNA. RNA sequencing was performed for mechanistic
analysis. The interaction between RBPMS2 and BMP pathway was studied using BMP inhibitor
and activator. The effect of the localization of E-cadherin was determined by immunofluorescence
staining.
Results
The maternal protein RBPMS2 is highly expressed in mouse oocytes and knockdown of
RBPMS2 inhibits embryo development from the morula to blastocyst stage. Mechanically,
RNA sequencing showed the differentially expressed genes were enriched in TGF-β signaling
pathway. BMPs are members of the TGF-β superfamily growth factors. We found that addition
of BMP inhibitor in KSOM medium led to morula stage arrest, similar to that seen in
RBPMS2 knockdown embryos. And the morula-stage arrest defect caused by RBPMS2 knockdown
was partially rescued by BMP activator. Furthermore, localization of E-cadherin in
the membrane was impaired in response to knockdown of RBPMS2 or inhibition of BMP pathway.
Conclusion
Our study suggests that RBPMS2 activates the BMP pathway and thus influences the localization
of E-cadherin, which is important for early mouse embryo development during blastocyst
formation.
Graphical abstract
Graphical Abstract
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Reproductive BioMedicine OnlineAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
Biography
Cheng Zhou, Master of Medicine, is Gynaecologist-in-charge in Department of Reproductive Medicine, Affiliated Jinling Hospital, Nanjing Medical University.
keymessage
Most of maternal factors that accumulate and stored in the cytoplasm of mature oocytes are still unknown. We evaluated maternal RBPMS2 protein activated the BMP pathway and influenced the localization of E-cadherin on cytomembrane, participating in the process of mouse blastocyst formation.
Article info
Publication history
Accepted:
May 18,
2023
Received in revised form:
April 24,
2023
Received:
November 16,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2023 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
