Maternal RBPMS2 protein is involved in mouse blastocyst formation through BMP pathway


      Research question

      Does RNA binding protein with multiple splicing 2 (RBPMS2), a maternal factor that accumulate and stored in the cytoplasm of mature oocytes, influenced early embryo development in mice?


      The expression pattern of rbpms2 in mouse were analyzed by qRT-PCR and immunofluorescence staining. The effect of knockdown of RBPMS2 on embryo development was evaluated through microinjection of specific morpholino or siRNA. RNA sequencing was performed for mechanistic analysis. The interaction between RBPMS2 and BMP pathway was studied using BMP inhibitor and activator. The effect of the localization of E-cadherin was determined by immunofluorescence staining.


      The maternal protein RBPMS2 is highly expressed in mouse oocytes and knockdown of RBPMS2 inhibits embryo development from the morula to blastocyst stage. Mechanically, RNA sequencing showed the differentially expressed genes were enriched in TGF-β signaling pathway. BMPs are members of the TGF-β superfamily growth factors. We found that addition of BMP inhibitor in KSOM medium led to morula stage arrest, similar to that seen in RBPMS2 knockdown embryos. And the morula-stage arrest defect caused by RBPMS2 knockdown was partially rescued by BMP activator. Furthermore, localization of E-cadherin in the membrane was impaired in response to knockdown of RBPMS2 or inhibition of BMP pathway.


      Our study suggests that RBPMS2 activates the BMP pathway and thus influences the localization of E-cadherin, which is important for early mouse embryo development during blastocyst formation.

      Graphical abstract


      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Reproductive BioMedicine Online
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


      Cheng Zhou, Master of Medicine, is Gynaecologist-in-charge in Department of Reproductive Medicine, Affiliated Jinling Hospital, Nanjing Medical University.
      Most of maternal factors that accumulate and stored in the cytoplasm of mature oocytes are still unknown. We evaluated maternal RBPMS2 protein activated the BMP pathway and influenced the localization of E-cadherin on cytomembrane, participating in the process of mouse blastocyst formation.