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Ultraviolet radiation dose
Reproductive BioMedicine OnlineVol. 22Issue 5p503Published online: February 14, 2011- Lodovico Parmegiani
- Graciela Estela Cognigni
- Marco Filicori
Cited in Scopus: 2With regard to the response of Isachenko (2011) to our letter ‘Efficacy of ultraviolet sterilization of liquid nitrogen’, we would like to point out that in the studies cited by Professor Isachenko the ultraviolet (UV) dose administered was too low for the complete inactivation of the microorganisms. Furthermore, the extremely radiation-resistant bacterium Deinococcus radiodurans is inactivated (>−4 log) by administering 400,000 μWs/cm2 (Krisko and Radman, 2010). Hence, an adequate amount of UV radiation de-activates the growth of all kinds of microorganisms. - Letter
Efficacy of ultraviolet sterilization of liquid nitrogen
Reproductive BioMedicine OnlineVol. 22Issue 5p501Published online: September 1, 2010- Lodovico Parmegiani
- Graciela Estela Cognigni
- Marco Filicori
Cited in Scopus: 1We read with interest the commentary of Isachenko et al. (2010) in this journal. The authors stated that vitrification methods that require direct plunging of ovarian tissue into liquid nitrogen (LN2) cannot be recommended for use in medical practice because these protocols presuppose a direct contact with the LN2, which is a potential source of microbial contamination. Furthermore, they said that UV treatment of LN2 cannot guarantee the absence of contamination of biological material by viruses.
